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Understanding the infection by the bacterium Listeria monocytogenes as a way to address key issues in biology

Understanding the infection by the bacterium Listeria monocytogenes as a way to address key issues in biology

Objective

Host-pathogen interactions have recently received much attention. Yet, a more comprehensive understanding of these interplays should provide fundamental advances in biology and help generating new therapeutics. The intracellular bacterium Listeria monocytogenes has emerged as an exceptional model organism to address key questions in biology such as actin-based motility, phagocytosis and post-transcriptional regulation. We will exploit our deep knowledge of Listeria and use this bacterium as a model to explore new landmarks in biology.
In that aim, we will analyze several aspects of the infectious process, in a spatio-temporal fashion, at the bacteria, cell, tissue and host levels. Specifically, we will 1) search and analyze the function of new non-coding RNAs involved in virulence, and of new RNA-mediated regulations; 2) investigate new facets of bacterial entry and cell-to-cell spread in particular the role of uncharacterized cytoskeletal components, septins; 3) address the role of mitochondria and their dynamics in infection; 4) systematically analyze post-translational modifications during infection, starting with SUMOylation, a reversible modification and proteolysis, an irreversible one; 5) investigate chromatin remodeling upon infection; 6) characterize new virulence factors identified by their interaction with known signaling components or cellular sensors, by post genomics or by their effect on cellular responses analyzed herein ; 7) investigate further the intestinal phase of Listeria infection by analyzing in the germ-free transgenic mouse that we generated, the impact of commensals on Listeria (growth and transcription including non-coding RNAs) and on the intestinal tissue (histology and transcription of genes and microRNAs).
Our main goal is to improve significantly our understanding of bacterial infections, by discovering important new concepts in microbiology, cell biology and infection biology thereby opening new avenues for further research.
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Principal Investigator

Pascale Cossart (Prof.)

Host institution

INSTITUT PASTEUR

Address

Rue Du Docteur Roux 25-28
75724 Paris Cedex 15

France

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 1 800 000

Principal Investigator

Pascale Cossart (Prof.)

Administrative Contact

Marie-Laure Rosso (Ms.)

Beneficiaries (1)

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INSTITUT PASTEUR

France

EU Contribution

€ 1 800 000

Project information

Grant agreement ID: 233348

Status

Closed project

  • Start date

    1 June 2009

  • End date

    31 May 2015

Funded under:

FP7-IDEAS-ERC

  • Overall budget:

    € 1 800 000

  • EU contribution

    € 1 800 000

Hosted by:

INSTITUT PASTEUR

France