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Single-Molecule Fluorescence Pulsed Interleaved Excitation, a novel tool to study biomolecular interactions

Single-Molecule Fluorescence Pulsed Interleaved Excitation, a novel tool to study biomolecular interactions

Objective

During the last few years, single-molecule fluorescence (SMF) methodologies have revolutionized the accessible information on biomolecular complexes, molecular dynamics, and biological mechanisms. Novel and important structural and dynamic insights can be obtained by studying molecules one by one, particularly into biological processes. In this field, the development of new methodologies and tools, on one hand, and of new improved fluorophores, on the other, are two major lines of research. In the current proposal, a cutting edge SMF methodology will be implemented: SMF Pulsed Interleaved Excitation (PIE) with two-colour coincidence detection, a technique that allows alternating excitation of fluorophores of different colours acting as donor and acceptor in fluorescence resonance energy transfer (FRET) processes. SMF-PIE has several advantages over the conventional SMF schemes to study biological systems, such as accurate determination of FRET efficiencies and inter-fluorophore distances, effective removal of the spectral, or distinction between molecules that have a low FRET efficiency from those that lack acceptor tag. The instrument performance will be calibrated and validated using model samples. Subsequently, SMF-PIE will be applied in three multidisciplinary biophysical approaches: 1) testing the feasibility and performance of newly synthesised fluorescein derivatives for SMF studies; 2) the resonance energy transfer between the new dyes acting as FRET donors and a DNA intercalator fluorophore as acceptor will provide insights into the mechanisms of intercalation of these carcinogenic molecules; 3) SMF-PIE will be employed to study the early pre-fibrillar aggregates from an amyloidogenic protein, the SH3 domain of α-spectrin, providing insights into the amyloid fibril formation mechanisms, a major biophysical and biomedical challenge nowadays.

Coordinator

UNIVERSIDAD DE GRANADA

Address

Cuesta Del Hospicio Sn
18071 Granada

Spain

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 45 000

Administrative Contact

Jose Maria Alvarez Pez (Prof.)

Project information

Grant agreement ID: 230917

Status

Closed project

  • Start date

    10 December 2008

  • End date

    9 December 2011

Funded under:

FP7-PEOPLE

  • Overall budget:

    € 45 000

  • EU contribution

    € 45 000

Coordinated by:

UNIVERSIDAD DE GRANADA

Spain