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Intestinal, Liver and Endothelial Nanoparticle Toxicity Development and evaluation of a novel tool for high-throughput data generation

Objective

The InLiveTox project will form an interdisciplinary consortium at the European level, together with a key American research group to develop an improved in vitro model for the study of nanoparticle (NP) uptake, transport and cellular interaction, thus advancing our understanding of NP toxicity. Rather than repeat what has, or is being done in the field of aerosol NP and lung toxicology, InLiveTox will focus on the impact of NP exposure via ingestion, in the healthy and diseased gastrointestinal (GI) tract, vascular endothelium and liver. The key questions in this study are: (i) How do these tissues individually respond to NPs? (ii) How do the interactions between the different tissues modulate their responses? (iii) How does inflammation affect the toxicity of NPs and their ability cross the intestinal barrier? (iv) Which physico-chemical characteristics of NPs influence their uptake by intestinal epithelial cells and their subsequent interactions with endothelial and liver cells? The objective of InLiveTox will be to develop a novel modular microfluidics-based in vitro test system modelling the response of cells and tissues to the ingestion of NPs. Cell culture modules of target tissues such as the GI tract, the liver and the endothelium will be connected via a microfluidics system so that knock-on and cross talk effects between organs and tissues can be monitored. A major innovative aspect of the InLiveTox project pertains to the implementation of biological tissue models in a microfabricated compartmental cell culture system that allows multiple cell types to be addressed and investigated in combination. This system will be much easier, more convenient and ethically less questionable than animal testing, as well as more relevant than the in vitro single cell /co-culture models currently used. For this study, applications of the model will focus on NP toxicology, but the system could also be widely used in various applications of toxicology and pharmacology.

Field of science

  • /social sciences/social and economic geography/transport
  • /medical and health sciences/basic medicine/pharmacology and pharmacy
  • /medical and health sciences/basic medicine/toxicology

Call for proposal

FP7-NMP-2008-SMALL-2
See other projects for this call

Funding Scheme

CP-FP - Small or medium-scale focused research project

Coordinator

CSEM CENTRE SUISSE D'ELECTRONIQUE ET DE MICROTECHNIQUE SA - RECHERCHE ET DEVELOPPEMENT
Address
Rue Jaquet Droz 1
2000 Neuchatel
Switzerland
Activity type
Research Organisations
EU contribution
€ 609 460
Administrative Contact
André Laville (Mr.)

Participants (8)

UNIVERSITA DI PISA
Italy
EU contribution
€ 364 552
Address
Lungarno Pacinotti 43/44
56126 Pisa
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Arti Ahluwalia (Prof.)
EDINBURGH NAPIER UNIVERSITY

Participation ended

United Kingdom
EU contribution
€ 162 821,60
Address
Colinton Road 219
EH14 1DJ Edinburgh
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Colette Pemberton (Ms.)
UNIVERSITAT DES SAARLANDES
Germany
EU contribution
€ 446 420
Address
Campus
66123 Saarbrucken
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Corinna Hahn (Ms.)
HELMHOLTZ ZENTRUM MUENCHEN DEUTSCHES FORSCHUNGSZENTRUM FUER GESUNDHEIT UND UMWELT GMBH
Germany
EU contribution
€ 441 437
Address
Ingolstadter Landstrasse 1
85764 Neuherberg
Activity type
Research Organisations
Administrative Contact
Jürgen Ertel (Dr.)
KIRKSTALL LIMITED
United Kingdom
EU contribution
€ 93 015
Address
York House, Outgang Lane, Osbaldwic, York
YO19 5UP North Yorkshire
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
John Malcolm Wilkinson (Dr.)
UNIVERSITY OF ROCHESTER
United States
Address
Elmwood Avenue, Box Ehsc 601
14642 Rochester Ny
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Günter Oberdörster (Prof.)
AYMING
France
EU contribution
€ 102 825
Address
185 Avenue Des Gresillons
92622 Gennevilliers Cedex
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Raffaella Catena (Dr.)
HERIOT-WATT UNIVERSITY
United Kingdom
EU contribution
€ 179 458,40
Address
Riccarton
EH14 4AS Edinburgh
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Eva Olszewska-Day (Dr.)