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Development of a high sensitive and specific nanobiosensor based on surface enhanced vibrational spectroscopy dedicated to the in vitro proteins detection and disease diagnosis

Objective

The main goal of our proposal is to develop a novel optical nanobiosensor based on extraordinary vibrational signal enhancement of the proteins to be detected. To reach vibrational signal enhancement, we will exploit the optical properties of specially designed metallic nanoparticles which should act as nanoantenna and the associated field enhancement to obtain a direct detection of proteins bound to the nanoparticle. Thus, our sensor will reach high sensitivity provided by the recently established large enhancement of vibration signals due to the resonant excitation of the nanoantenna device used as substrates. The aim is to detect only a few proteins with concentration much lower than 1pM and finally to reach detection threshold such as femtomole or lower. High molecular selectivity will be reached with the functionalisation of the nanoantenna. Such functionalisation will selectively favour the immobilisation of the protein to be detected at the vicinity of the nanoparticle surface, providing the best enhancement and then the detection of the targeted protein. Our nanobiosensor will include two main components: the nanoantenna device which corresponds to our sensor transducer and the functionalisation which corresponds to its bioreceptor. And then, each functionalised nanoantenna device used as vibrational signal enhanced system is an individual and specific nanosensor of proteins. As a consequence, our nanobiosensor integrated in a vibrational spectroscope will allow the detection and the analysis of the enhanced vibrational signal from the targeted proteins and thus corresponds to our diagnosis instrument. Our nanobiosensor will be validated on the detection of proteins on body fluids.These proteins have been chosen since they have been identified as specific biomarkers of common pathologies. This validation will be applied it to improve their detection (better sensitivity, decrease of the detection threshold) and open the way to the early diagnosis.

Field of science

  • /medical and health sciences/basic medicine/pathology
  • /natural sciences/chemical sciences/analytical chemistry/spectroscopy
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins

Call for proposal

FP7-HEALTH-2009-single-stage
See other projects for this call

Funding Scheme

CP-FP - Small or medium-scale focused research project

Coordinator

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
Address
Rue Michel Ange 3
75794 Paris
France
Activity type
Research Organisations
EU contribution
€ 599 491
Administrative Contact
Gilles Traimond (Mr.)

Participants (11)

AGENCIA ESTATAL CONSEJO SUPERIOR DEINVESTIGACIONES CIENTIFICAS
Spain
EU contribution
€ 279 891,50
Address
Calle Serrano 117
28006 Madrid
Activity type
Research Organisations
Administrative Contact
Carlos M. Abad Ruiz (Mr.)
INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE
France
EU contribution
€ 277 718,30
Address
Rue De Tolbiac 101
75654 Paris
Activity type
Research Organisations
Administrative Contact
Nathalie Charnaux (Dr.)
RUPRECHT-KARLS-UNIVERSITAET HEIDELBERG
Germany
EU contribution
€ 319 946
Address
Seminarstrasse 2
69117 Heidelberg
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Verena Schultz-Coulon (Dr.)
CONSIGLIO NAZIONALE DELLE RICERCHE
Italy
EU contribution
€ 282 165
Address
Piazzale Aldo Moro 7
00185 Roma
Activity type
Research Organisations
Administrative Contact
Giovanna Toscano (Ms.)
BEN-GURION UNIVERSITY OF THE NEGEV
Israel
EU contribution
€ 319 897
Address
.
84105 Beer Sheva
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Daphna Tripto (Ms.)
ASOCIACION CENTRO DE INVESTIGACIONCOOPERATIVA EN NANOCIENCIAS CIC NANOGUNE
Spain
EU contribution
€ 270 036
Address
Tolosa Hiribidea 76
20018 San Sebastian
Activity type
Research Organisations
Administrative Contact
Miguel Odriozola Braconier (Mr.)
FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA
Italy
EU contribution
€ 340 131
Address
Via Morego 30
16163 Genova
Activity type
Research Organisations
Administrative Contact
Gabriele Ballero (Mr.)
HORIBA FRANCE SAS
France
EU contribution
€ 367 092
Address
Rue Du Canal 16-18
91160 Longjumeau
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Blandine Mariot (Ms.)
ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES- CIC biomaGUNE
Spain
EU contribution
€ 269 649
Address
Paseo Miramon 182, Parque Tecnologico De San Sebastian Edificio Empresarial C
20009 San Sebastian
Activity type
Research Organisations
Administrative Contact
Valery Pavlov (Dr.)
UNIVERSITE DE TECHNOLOGIE DE TROYES
France
EU contribution
€ 273 950
Address
Rue Marie Curie 12
10004 Troyes
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Dominique Barchiesi (Prof.)
TECHNOCLONE HERSTELLUNG DIAGNOSTIKAUND ARZNEIMITTELN GMBH
Austria
EU contribution
€ 399 959
Address
Brunnerstrasse 59/5
1230 Wien
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Veronika Binder (Mrs.)