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Health Impact of Engineered Metal and Metal Oxide Nanoparticles: Response, Bioimaging and Distribution at Cellular and Body Level

Objective

Metal oxide and metal NPs are particularly dangerous for two reasons: their special catalytic activity coming from the properties of their nanointerface may interfere with numerous intracellular biochemical processes and the decomposition of NPs and the ion leakage could heavily interfere with the intracellular free metal ion homeostasis, which is essential for cell metabolism. A very specific problem is the difficulty of localizing and quantifying them in cells. Obtaining dose effect relationships is not simple, because of the unknown amount of material present in affected cells. The following main points will be addressed in this proposal:1) Design and synthesis of metal oxide and metal NPs, which can be traced by SPECT, PET, and fluorescence techniques and the appropriate characterization of these NPs.2) Application of label-free techniques, such as IBM and EM to ensure that the radioactive and fluorescent constituents do not modify the cytological and organismic response by themselves.3) Characterization of the uptake, distribution kinetics and NP release at the level of the organism.4) Study of the interaction of NPs with plasma components forming complexes with NPs and the assessment of their possible impact on the uptake compared with that of bare or capped particles.5) Quantification and localization of metallic NPs in immune competent cells is a key task for the establishment of proper dose-response correlations. A technique applicable with living cells as ultimate control will be IBM, capable of detecting single metal NPs in cells at different depths.6) Development of sophisticated cell physiological approaches focusing on the determination of oxidative activity, cytokine production and adaptive processes concerning signalling pathways beyond standard vitality tests. The research project will indicate toxic levels of various NPs and sub-toxic effects will be investigated by analysing the signalling response of immune cells
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Coordinator

ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES- CIC biomaGUNE

Address

Paseo Miramon 182, Parque Tecnologico De San Sebastian Edificio Empresarial C
20009 San Sebastian

Spain

Activity type

Research Organisations

EU Contribution

€ 506 880,33

Administrative Contact

Sergio Enrique Moya (Dr.)

Participants (8)

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UNIVERSIDAD DE VIGO

Spain

EU Contribution

€ 339 678,02

UNIVERSITAET LEIPZIG

Germany

EU Contribution

€ 511 730,40

EDUCACAO E ENSINO SUPERIOR DO ALTO AVE S.A.

Portugal

CENTRO DE INVESTIGACION EN QUIMICA APLICADA

Mexico

EU Contribution

€ 110 499,38

Zhejiang University

China

EU Contribution

€ 98 141,27

PLASMACHEM PRODUKTIONS- UND HANDEL GMBH

Germany

EU Contribution

€ 241 200

DET NATIONALE FORSKNINGSCENTER FORARBEJDSMILJO

Denmark

EU Contribution

€ 330 108

TYOTERVEYSLAITOS

Finland

EU Contribution

€ 159 099,60

Project information

Grant agreement ID: 228825

Status

Closed project

  • Start date

    1 October 2009

  • End date

    30 September 2012

Funded under:

FP7-NMP

  • Overall budget:

    € 2 927 577,99

  • EU contribution

    € 2 297 337

Coordinated by:

ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES- CIC biomaGUNE

Spain