Objectif Plants have the ability to form new organs according to an indeterminate growth that is ensured by pluripotent cells in organized structures called meristems. During floral induction the shoot apical meristem is converted into an inflorescence meristem, which gives rise to floral meristems (FM) leading to flower formation. This process called floral termination is under the control of the meristematic organizer gene WUSCHEL (WUS). The meristematic activity is intimately linked to hormonal signaling. However little is known about the integration of multi-hormonal signals during floral development. Recently, MIni Zinc Finger (MIF) proteins have been characterized in Arabidopsis, and shown to be involved in multiple hormonal regulations during plant development. Previously we isolated a gene named INHIBITOR OF MERISTEM ACTIVITY (IMA) as a tomato MIF ortholog. IMA was shown to regulate meristem activity by inhibiting the expression of WUSCHEL. Tomato plants overexpressing IMA exhibit a dwarf phenotype suggesting that IMA regulates negatively cell proliferation and expansion. A Two-Hybrid screen contributed to identify the COP9-signalosome subunit 5, as a strong interacting protein. The COP9-signalosome plays a conserved key role in the integration of endogenous and exogenous signals to regulate growth of eukaryotic organisms, including cancer development in human when a deregulation of this complex occurs. Our data suggest that IMA controls COP9-signalosome activity to modulate multihormonal responses during meristem development and floral termination. This project aims at understanding the molecular and genetic mechanisms by which IMA and more generally MIF proteins are involved in such regulatory pathways. In the context of fleshy fruit development, this innovative project will promote fundamental knowledge on an original model, but also will generate agronomical benefits through applications at the breeding and biotechnological level for fruit quality improvement. Champ scientifique natural scienceschemical sciencesinorganic chemistrytransition metalsagricultural sciencesagriculture, forestry, and fisheriesagriculturehorticulturefruit growingnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesclinical medicineoncology Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) FP7-PEOPLE-2009-RG - Marie Curie Action: "Reintegration Grants" Appel à propositions FP7-PEOPLE-2009-RG Voir d’autres projets de cet appel Régime de financement MC-IRG - International Re-integration Grants (IRG) Coordinateur INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT Contribution de l’UE € 50 000,00 Adresse 147 RUE DE L'UNIVERSITE 75007 Paris France Voir sur la carte Région Ile-de-France Ile-de-France Paris Type d’activité Research Organisations Contact administratif Christian Chevalier (Dr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée
