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Protein Binders for Characterisation of Human Proteome Function: Generation, Validation, Application

Objective

The Affinomics programme aims to leverage existing efforts in Europe to generate large-scale resources of validated protein-binding molecules (‘binders’) as affinity reagents for characterisation of the human proteome and to apply them in comprehensive structural and functional analyses of protein expression, interactions and complexes. Proteome targets will be focused on five categories of inter-related human proteins involved in signal transduction, cell regulation and cancer, namely protein kinases, SH2 domain-containing proteins, protein tyrosine phosphatases, proteins somatically mutated in cancers and candidate cancer biomarkers. Binders to about 1000 protein targets will be made over the course of the programme. A high throughput, coordinated production pipeline for antigens and binders will be established. Target antigens will be expressed in three forms, as folded full-length proteins or domains, as large peptide fragments (PrESTs) based on low homology to other human proteins and as small peptides, in some cases phosphorylated. Binder types to be generated include affinity-purified polyclonal antibodies, monoclonal antibodies, recombinant antibody fragments and non-immunoglobulin scaffolds. An important aspect will be the development of highly efficient ‘next generation’ recombinant selection methods, based on phage, cell and ribosome display, capable of producing high quality binders at greater throughput and lower cost than hitherto. Systems and procedures for thorough binder validation and quality control will be established. The affinity reagents will be applied in advanced innovative and sensitive technologies for specific detection of target proteins and interacting protein complexes in cells, tissues and fluids, for improved understanding of protein function and new classes of diagnostic assays.

Field of science

  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins
  • /medical and health sciences/clinical medicine/oncology/cancer
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins/proteomics

Call for proposal

FP7-HEALTH-2009-two-stage
See other projects for this call

Funding Scheme

CP-IP - Large-scale integrating project

Coordinator

THE BABRAHAM INSTITUTE
Address
Babraham Hall
CB22 3AT Cambridge
United Kingdom
Activity type
Research Organisations
EU contribution
€ 823 536
Administrative Contact
Fang Gao (Mrs.)

Participants (19)

KUNGLIGA TEKNISKA HOEGSKOLAN
Sweden
EU contribution
€ 1 909 515
Address
Brinellvagen 8
100 44 Stockholm
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Eriksson åke (Mr.)
UNIVERSITAT ZURICH
Switzerland
EU contribution
€ 1 125 484
Address
Ramistrasse 71
8006 Zürich
Activity type
Other
Administrative Contact
Andreas Plückthun (Prof.)
MAX IV Laboratory, Lund University
Sweden
EU contribution
€ 695 742
Address
Paradisgatan 5C
22100 Lund
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Carl Borrebaeck (Prof.)
KOBENHAVNS UNIVERSITET
Denmark
EU contribution
€ 727 600
Address
Norregade 10
1165 Kobenhavn
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Ivan Kristoffersen (Mr.)
TEKNOLOGIAN TUTKIMUSKESKUS VTT
Finland
EU contribution
€ 459 007
Address
Tekniikantie 4 A
02044 VTT Espoo
Activity type
Research Organisations
Administrative Contact
Jaana Heino (Ms.)
EUROPEAN MOLECULAR BIOLOGY LABORATORY
Germany
EU contribution
€ 454 400
Address
Meyerhofstrasse 1
69117 Heidelberg
Activity type
Research Organisations
Administrative Contact
Barbara Baron (Ms.)
UNIVERSITA DEGLI STUDI DI ROMA TOR VERGATA
Italy
EU contribution
€ 398 282
Address
Via Cracovia 50
00133 Roma
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Gianni Cesareni (Prof.)
BABRAHAM BIOSCIENCE TECHNOLOGIES LIMITED
United Kingdom
EU contribution
€ 220 672
Address
Babraham Hall
CB22 3AT Cambridge
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Rebecca Paxton (Ms.)
TECHNISCHE UNIVERSITAET BRAUNSCHWEIG
Germany
EU contribution
€ 565 664
Address
Universitaetsplatz 2
38106 Braunschweig
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Stefan Dübel (Prof.)
UNIVERSITAET KASSEL
Germany
EU contribution
€ 426 579
Address
Monchebergstrasse 19
34125 Kassel
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Friedrich Herberg (Prof.)
HELMHOLTZ ZENTRUM MUENCHEN DEUTSCHES FORSCHUNGSZENTRUM FUER GESUNDHEIT UND UMWELT GMBH
Germany
EU contribution
€ 131 571
Address
Ingolstadter Landstrasse 1
85764 Neuherberg
Activity type
Research Organisations
Administrative Contact
Jürgen Ertel (Dr.)
STRUCTURAL GENOMICS CONSORTIUM LBG
United Kingdom
Address
Euston Road 215
NW1 2BE London
Activity type
Research Organisations
Administrative Contact
Susanne Muller-Knapp (Dr.)
DEUTSCHES KREBSFORSCHUNGSZENTRUM HEIDELBERG
Germany
EU contribution
€ 540 358
Address
Im Neuenheimer Feld 280
69120 Heidelberg
Activity type
Research Organisations
Administrative Contact
Bettina Crispin (Ms.)
VIB VZW
Belgium
EU contribution
€ 505 473
Address
Rijvisschestraat 120
9052 Zwijnaarde - Gent
Activity type
Research Organisations
Administrative Contact
Rik Audenaert (Mr.)
KAROLINSKA INSTITUTET
Sweden
EU contribution
€ 290 763
Address
Nobels Vag 5
17177 Stockholm
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Miles Davies (Dr.)
THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD
United Kingdom
EU contribution
€ 290 763
Address
Wellington Square University Offices
OX1 2JD Oxford
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Stephen Conway (Dr.)
EBERHARD KARLS UNIVERSITAET TUEBINGEN
Germany
EU contribution
€ 374 014
Address
Geschwister-scholl-platz
72074 Tuebingen
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Christine Augustin
UPPSALA UNIVERSITET
Sweden
EU contribution
€ 670 293
Address
Von Kraemers Alle 4
751 05 Uppsala
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Margareta Uvhagen Antoni (Ms.)
PARATOPES LIMITED
United Kingdom
EU contribution
€ 390 284
Address
Woodfall Avenue 62 Barnet
EN5 2HA London
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Alan Sawyer (Mr.)