CORDIS
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Application of massive parallel sequencing to microRNA profiling of cellular and molecular response to HIV-1 and HIV-2 infection and to modulation of integration

Application of massive parallel sequencing to microRNA profiling of cellular and molecular response to HIV-1 and HIV-2 infection and to modulation of integration

Objective

miRNAs are key regulators of cell function and pathogen infection. The identification of miRNAs involved inHIV infection promises to offer unique insights into disease mechanisms and to help identify novel targets for therapy. However, the ability to perform an unbiased characterization of miRNA expression has only recently become possible through the use of massive parallel sequencing. This proposal aims to generate the first whole-genome map of miRNA expression during HIV-1 and HIV-2 infection in primary lymphocytes using these novel sequencing techniques. Furthermore, we will identify miRNAs associated with key steps of infection by combining sequencing with the use of drug inhibitors that block viral DNA integration. We expect to identify miRNAs required for the progression of viral infection, with potential for future use in anti-HIV therapy. Pathways associated with differential miRNA expression between HIV-1 and HIV-2 will provide insights into the mildness of HIV-2 infection, providing clues for new strategies against HIV-1.
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Coordinator

FUNDACAO DA FACULDADE DE CIENCIAS DA UNIVERSIDADE DE LISBOA FP

Address

Campo Grande Edificio C1 Piso 3
1749 016 Lisboa

Portugal

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 45 000

Administrative Contact

Livia Moreira (Ms.)

Project information

Grant agreement ID: 256595

Status

Closed project

  • Start date

    1 July 2010

  • End date

    29 November 2013

Funded under:

FP7-PEOPLE

  • Overall budget:

    € 45 000

  • EU contribution

    € 45 000

Coordinated by:

FUNDACAO DA FACULDADE DE CIENCIAS DA UNIVERSIDADE DE LISBOA FP

Portugal