Dissecting the biogenesis of eukaryotic ribosomal subunits

Projektinformationen

EURIBIO

ID Finanzhilfevereinbarung: 260676

Abgeschlossenes Projekt

Startdatum 1 September 2010

Enddatum 31 Juli 2016

Finanziert unter

• Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Gesamtkosten € 1 500 000,00

EU-Beitrag € 1 500 000,00

Koordiniert durch

EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
Switzerland

Ziel

In all living cells, the important task of protein synthesis is carried out by the ribosome. A substantial amount of cellular energy and resources is utilized to manufacture ribosomal subunits. In contrast to prokaryotes, eukaryotic ribosome assembly requires a multitude of conserved non-ribosomal trans-acting factors, which transiently associate with pre-ribosomal particles at distinct assembly stages and perform specific maturation steps.
Large-scale proteomic approaches in budding yeast, have rapidly expanded the inventory of trans-acting factors (~200). However, little is known regarding their precise site(s) of action and the role(s) of these factors during pre-ribosome assembly. Upon accomplishing their task, majority of the trans-acting factors, are typically released from maturing pre-ribosomes already in the nucleolus/nucleus. Strikingly, a handful of factors remain associated with pre-ribosomes and facilitate their export into the cytoplasm. Release of these factors constitutes “late cytoplasmic maturation events” which render exported pre-ribosomes translation competent. In this proposal we will exploit the powerful model organism budding yeast to:
(1) Develop novel biochemical tools to elucidate the molecular environment of trans-acting factors on the surface of pre-ribosomal particles. These analyses will provide us a low-resolution biochemical map of a maturing pre-ribosome.
(2) Exploit the powerful combination of genetic and high-throughput visual screening approaches in budding yeast to unravel novel “late cytoplasmic maturation steps” in the 60S biogenesis pathway.

Together, my research proposal aims to contribute significantly to our current knowledge regarding the construction and nuclear export of eukaryotic pre-ribosomes. Our analysis will lead us to general principles that underlie the dynamic assembly/dissassembly of large macromolecular ribonucleo-protein complexes.

Wissenschaftliches Gebiet

• natural sciences › biological sciences › biochemistry › biomolecules › proteins

Programm/Programme

• FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Thema/Themen

• ERC-SG-LS1 - ERC Starting Grant - Molecular and Structural Biology and Biochemistry

Aufforderung zur Vorschlagseinreichung

ERC-2010-StG_20091118
Andere Projekte für diesen Aufruf anzeigen

Finanzierungsplan

ERC-SG - ERC Starting Grant

Gastgebende Einrichtung

EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH

Kontakt Verwaltung

Vikram Govind Panse (Prof.)

Hauptforscher

Vikram Govind Panse (Prof.)

• EU-Beitrag: € 1 500 000,00
• Adresse: Raemistrasse 101 ; 8092 Zuerich ; Schweiz
• Region: Schweiz/Suisse/Svizzera › Zürich › Zürich
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: Keine Daten

Begünstigte (1)

EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH

Schweiz

Kontakt Verwaltung

Vikram Govind Panse (Prof.)

Hauptforscher

Vikram Govind Panse (Prof.)

• EU-Beitrag: € 1 500 000,00
• Adresse: Raemistrasse 101 ; 8092 Zuerich
• Region: Schweiz/Suisse/Svizzera › Zürich › Zürich
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: Keine Daten