Single Molecule Enzymology with ClyA Nanopores

Projektinformationen

SMEN

ID Finanzhilfevereinbarung: 260884

Abgeschlossenes Projekt

Startdatum 1 November 2010

Enddatum 31 Oktober 2016

Finanziert unter

• Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Gesamtkosten € 1 220 473,60

EU-Beitrag € 1 220 473,60

Koordiniert durch

RIJKSUNIVERSITEIT GRONINGEN
Netherlands

Ziel

Single molecules based techniques provide the ultimate toolkit to study complex biological systems. In single-molecule approaches, molecules do not need to be synchronised as in ensemble studies, and rare and/or transient species along a reaction pathway as well as heterogeneity and disorder in a sample can be revealed.
Observing and manipulating single molecules, however, is generally complicated, time consuming and withstand several technical limitations that hamper the study of single enzymes to a few selected examples.

Here I am proposing to develop a new technology to study single native enzymes that is sensitive, simple and inexpensive, and has a temporal resolution ranging from few ¼seconds to hours.

Nanopores have been used to detect single molecules and to investigate mechanisms of chemical reactions at the single molecule level. The basic concept of nanopore analysis is to observe, under an applied potential, the disruption of the flow of ions through the pore caused by the interaction of the molecules of interest with a binding site within the pore.
Similarly, small enzymes or functional nucleic acids will be attached to the vestibule of a biological nanopore via disulfide bridge or click chemistry. The conformational changes associated with catalysis will then be observed by the altered ionic flow through the pore. In addition, when a charged chaotropic agent is placed on the trans side of the bilayer, the applied potential will allow the directional control of the flow of the chaotropic agent through the pore that, ultimately, also will control the unfolding and refolding of the protein attached to the pore. This will allow investigations of reversible unfolding processes far from equilibrium at the single molecule level for the first time.

Wissenschaftliches Gebiet

• natural sciences › biological sciences › biochemistry › biomolecules › nucleic acids
• natural sciences › chemical sciences › catalysis
• natural sciences › biological sciences › biochemistry › biomolecules › proteins › enzymes

Programm/Programme

• FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Thema/Themen

• ERC-SG-LS9 - ERC Starting Grant - Applied life sciences and biotechnology

Aufforderung zur Vorschlagseinreichung

ERC-2010-StG_20091118
Andere Projekte für diesen Aufruf anzeigen

Finanzierungsplan

ERC-SG - ERC Starting Grant

Gastgebende Einrichtung

RIJKSUNIVERSITEIT GRONINGEN

Kontakt Verwaltung

Dick Veldhuis (Dr.)

Hauptforscher

Giovanni Maglia (Dr.)

• EU-Beitrag: € 451 215,26
• Adresse: Broerstraat 5 ; 9712CP Groningen ; Niederlande
• Region: Noord-Nederland › Groningen › Overig Groningen
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: Keine Daten

Begünstigte (2)

RIJKSUNIVERSITEIT GRONINGEN

Niederlande

Kontakt Verwaltung

Dick Veldhuis (Dr.)

Hauptforscher

Giovanni Maglia (Dr.)

• EU-Beitrag: € 451 215,26
• Adresse: Broerstraat 5 ; 9712CP Groningen
• Region: Noord-Nederland › Groningen › Overig Groningen
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: Keine Daten

KATHOLIEKE UNIVERSITEIT LEUVEN

Beteiligung beendet

Belgien

Kontakt Verwaltung

Myriam Witvrouw (Ms.)

• EU-Beitrag: € 769 258,34
• Adresse: OUDE MARKT 13 ; 3000 Leuven
• Region: Vlaams Gewest › Prov. Vlaams-Brabant › Arr. Leuven
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: Keine Daten