Objective A number of studies suggest that the larval imaginal discs, the precursors of the adult structures in the fly, play a crucial role in coordinating tissue maturation/patterning with developmental timing. Thus, disc transplantation experiments show that the transplantation of a young imaginal discs into more developmentally advanced larvae delays pupal molt, allowing the underdeveloped discs to catch up with other larval tissues. In contrast, complete ablation of the imaginal discs has no effect on developmental timing of the pupal molt. These results suggest that a signal released from imaginal discs prevents the onset of metamorphosis up until patterning/maturation of the discs is complete. Reminiscent of this, wounding of imaginal discs delays pupal molt, thereby allowing damaged tissue to regenerate before the onset of metamorphosis. So far, the mechanisms by which immature/wounded discs prevent the production of the steroid hormone ecdysone and thereby pupal molt remains elusive. The aim of the proposed project is to screen for the signals that couple patterning of mitotic tissue with the developmental clock using the power of Drosophila genetics. For this purpose we developed two different conditions for which we can induce a developmental delay by altering the expression of two selected genes specifically in the discs. These two conditions will be used at two successive levels to screen the VDRC collection of UAS-RNAi lines (12.000 lines covering 95% of the genes having a mammalian ortholog) for candidates that can revert or at least partially rescue the developmental delay. Fields of science natural sciencesbiological sciencesgeneticsmedical and health sciencesclinical medicinetransplantation Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) FP7-PEOPLE-2009-IEF - Marie Curie Action: "Intra-European Fellowships for Career Development" Call for proposal FP7-PEOPLE-2009-IEF See other projects for this call Funding Scheme MC-IEF - Intra-European Fellowships (IEF) Coordinator CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS EU contribution € 166 145,60 Address RUE MICHEL ANGE 3 75794 Paris France See on map Region Ile-de-France Ile-de-France Paris Activity type Research Organisations Administrative Contact Pierre Dauchez (Mr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data
