CORDIS
EU research results

CORDIS

English EN

In-vitro system for mammalian miRNA function

Project information

Grant agreement ID: 253415

Status

Closed project

  • Start date

    1 April 2010

  • End date

    31 March 2012

Funded under:

FP7-PEOPLE

  • Overall budget:

    € 159 661

  • EU contribution

    € 159 661

Coordinated by:

EUROPEAN MOLECULAR BIOLOGY LABORATORY

Germany

Objective

Over the past decade, micro (mi) RNAs have emerged as key regulators of gene expression. Many miRNAs have been linked to diseases, such as cancer or diabetes. MiRNAs bind to specific sequences in the 3’-untranslated region (UTR) of mRNAs, thereby causing inhibition of translation and/or mRNA degradation. However, the detailed molecular mechanism and the factors involved in miRNA-mediated gene silencing are still incompletely understood. The primary research objective of the current proposal is the generation of a novel mammalian in-vitro system that will allow a detailed biochemical dissection of the molecular mechanisms underlying medical relevant miRNA-guided gene silencing. In particular, we will characterize repression mediated by the tumorsuppressor miRNA let-7. We will elucidate the role of cis- and trans-acting factors in miRNA mediated repression and use the system to purify native miRISC-RNP-complexes (Ribonucleoprotein-complexes) and subsequently identify and characterize the components of the complex. Thus, the knowledge gained from this proposal will enhance our understanding of miRNA function and provide novel insights into the role of RNA metabolism in disease, which will be applicable to the development of novel diagnostic and therapeutic strategies targeting miRNA-associated pathologies.

Coordinator

EUROPEAN MOLECULAR BIOLOGY LABORATORY

Address

Meyerhofstrasse 1
69117 Heidelberg

Germany

Activity type

Research Organisations

EU Contribution

€ 159 661

Administrative Contact

Phil Irving (Dr.)

Project information

Grant agreement ID: 253415

Status

Closed project

  • Start date

    1 April 2010

  • End date

    31 March 2012

Funded under:

FP7-PEOPLE

  • Overall budget:

    € 159 661

  • EU contribution

    € 159 661

Coordinated by:

EUROPEAN MOLECULAR BIOLOGY LABORATORY

Germany