Objectif The objective of my laboratory is to understand the mechanisms of mRNA polyadenylation and deadenylation, thereby providing fundamental insight into the regulation of eukaryotic gene expression. These objectives will be met through an integrated, multidisciplinary approach combining structural, biochemical, biophysical and yeast genetic techniques. Specifically, I propose to establish how mRNA poly(A) tail position and length are determined by the Cleavage and Polyadenylation Factor (CPF) and by the Ccr4-Not complex.Gene expression is tightly regulated, both spatially and temporally. Traditionally, this has been studied through transcription but more recent work has shown that post-transcriptional mechanisms play extensive roles. Many of these post-transcriptional functions are mediated by poly(A) tails which are found on almost all eukaryotic mRNAs. The CPF and Ccr4-Not complexes influence poly(A) tails to regulate mRNA stability and the efficiency of translation. They also mediate the periodic expression of cell cycle-related genes, microRNA-targeted gene silencing and expression of maternal masked mRNAs in oocyte development. My work will address many of these functions and will have broad-ranging biological and technological implications including: a better understanding of gene expression and the newly-emerging field of post-transcriptional regulation, the development of new technologies to study multi-protein complexes and the identification of potential therapeutic targets. Programme(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) ERC-SG-LS1 - ERC Starting Grant - Molecular and Structural Biology and Biochemistry Appel à propositions ERC-2010-StG_20091118 Voir d’autres projets de cet appel Régime de financement ERC-SG - ERC Starting Grant Institution d’accueil MEDICAL RESEARCH COUNCIL Contribution de l’UE € 1 124 064,00 Adresse 20 Park Crescent W1B 1AL LONDON Royaume-Uni Voir sur la carte Type d’activité Public bodies (excluding Research Organisations and Secondary or Higher Education Establishments) Contact administratif Elizabeth Cutler (Ms.) Chercheur principal Lori Anne Passmore (Dr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée Bénéficiaires (1) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire MEDICAL RESEARCH COUNCIL Royaume-Uni Contribution de l’UE € 1 124 064,00 Adresse 20 Park Crescent W1B 1AL LONDON Voir sur la carte Type d’activité Public bodies (excluding Research Organisations and Secondary or Higher Education Establishments) Contact administratif Elizabeth Cutler (Ms.) Chercheur principal Lori Anne Passmore (Dr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée
