Cel Centrioles are cellular organelles made of microtubules that are essential for centrosome, cilia and flagella assembly and as such are involved in a variety of cellular and developmental processes, including cell motility, division and polarity. Centriole biogenesis is first seen during S phase by the appearance of procentrioles that elongate as the cell cycle progresses to reach nearly full length in mitosis. Most mature centrioles in an organism have similar length, yet very little is known on how centriole length control is exerted. The purpose of the current project is to investigate centriole biogenesis, in particular centriole elongation, its kinetics and regulation. We will search for novel molecular players in this process via two complementary approaches. We will perform an RNAi screening of microtubule regulators in mammalian cells and check for their role in centriole elongation. In addition, we will perform pull-down assays using already known molecular players in centriole length control and search for new binding partners by mass spectrometry. These combined approaches will identify novel players in centriole biogenesis that we will further study using in vitro and in vivo (Drosophila) approaches, to better understand their mechanism of action and their relevance to the organism. Dziedzina nauki natural scienceschemical sciencesanalytical chemistrymass spectrometry Program(-y) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Temat(-y) FP7-PEOPLE-2009-IEF - Marie Curie Action: "Intra-European Fellowships for Career Development" Zaproszenie do składania wniosków FP7-PEOPLE-2009-IEF Zobacz inne projekty w ramach tego zaproszenia System finansowania MC-IEF - Intra-European Fellowships (IEF) Koordynator FUNDACAO CALOUSTE GULBENKIAN Wkład UE € 148 783,60 Adres AVENIDA BERNA 45A 1067-001 LISBOA Portugalia Zobacz na mapie Region Continente Área Metropolitana de Lisboa Área Metropolitana de Lisboa Rodzaj działalności Research Organisations Kontakt administracyjny José Mario Leite (Mr.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych
