Objectif Legionella outbreaks have great sanitary, economic and social implications. Diagnostic method use to prevent and control Legionella is bacteria culture. Available detection kits are based on DNA technology, unable to discriminate between viable and non-viable bacteria. On the contrary, RNA is widely used as a viable bacteria indicator. Although molecular based techniques, as PCR and qPCR, have become essential tools in genetic analyses due to its high sensitivity and accuracy, they show drawbacks as time-consuming and expensive. Recent development of integrated microfluidic systems has allowed time, volume and analysis cost reduction. An important application of microfluididc systems is the ability to efficiently process raw samples and perform the required analytical assays “on-chip”. There is no portable system to detect viable Legionella on-site in the market. This device will avoid sample pre-treatment therefore reducing data acquisition. Moreover, rapid result gathering will allow implementing a rapid plan of action for bacteria eradication. The project aims to develop a portable microfluidic device composed of a filtration and bacteria collection system and a disposable card (containing a microfluidic chamber for nucleic acids concentration, a microfluidic chamber for RNA amplification through NASBA, and a detection system based on a NALFIA strip). An essential part of PINVIALEG is the development of a portable device where the filtration system is integrated to a microfluidic based cartridge. Thus, the whole process of Legionella detection (from sample collection to results) can be performed on site. NASBA is an isothermal process, facilitating portable equipment production. Development of a NALFIA strip able to detect NASBA products as a reading system will make the device very simple to use by non-qualified personnel. A new innovative tool will be produced to detect viable Legionella on-site, with rapid result acquisition and easy to use. Champ scientifique natural sciencesphysical sciencesclassical mechanicsfluid mechanicsmicrofluidicsnatural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acidsnatural sciencesbiological sciencesmicrobiologybacteriologynatural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesgeneticsRNA Programme(s) FP7-SME - Specific Programme "Capacities": Research for the benefit of SMEs Thème(s) SME-1 - Research for SMEs Appel à propositions FP7-SME-2010-1 Voir d’autres projets de cet appel Régime de financement BSG-SME - Research for SMEs Coordinateur ONDOAN S COOP LTDA Contribution de l’UE € 297 397,00 Adresse Parque Tecnológico. Ibaizabal bidea 101-C 48170 Zamudio Espagne Voir sur la carte Région Noreste País Vasco Bizkaia Type d’activité Private for-profit entities (excluding Higher or Secondary Education Establishments) Contact administratif Pedro Gurrutxaga (Mr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée Participants (7) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire MK FLUIDICS OY Finlande Contribution de l’UE € 319 325,00 Adresse Mataratie 2 A 1 90580 Oulu Voir sur la carte Type d’activité Private for-profit entities (excluding Higher or Secondary Education Establishments) Contact administratif Markku Tapani Känsäkoski (Mr.) Liens Contacter l’organisation Opens in new window Coût total Aucune donnée INNOSIEVE DIAGNOSTICS BV Pays-Bas Contribution de l’UE € 247 513,50 Adresse CELSIUSLAAN 5 5251 ZB Vlijmen Voir sur la carte Région Zuid-Nederland Noord-Brabant Noordoost-Noord-Brabant Type d’activité Private for-profit entities (excluding Higher or Secondary Education Establishments) Contact administratif Michel Klerks (Dr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée Q-BIOANALYTIC GMBH Allemagne Contribution de l’UE € 261 659,20 Adresse FISCHKAI 1 27572 BREMERHAVEN Voir sur la carte Type d’activité Private for-profit entities (excluding Higher or Secondary Education Establishments) Contact administratif Boris Oberheitmann (Dr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée AYUNTAMIENTO DE BILBAO Espagne Contribution de l’UE € 8 600,30 Adresse PLAZA ERNESTO ERKOREKA 1 48007 Bilbao Voir sur la carte Région Noreste País Vasco Bizkaia Type d’activité Public bodies (excluding Research Organisations and Secondary or Higher Education Establishments) Contact administratif Marina Lizarralde (Ms.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée FUNDACION GAIKER Espagne Contribution de l’UE Aucune donnée Adresse PARQUE TECNOLOGICO DE VIZCAYA, EDIFICIO 202 48170 Zamudio Voir sur la carte Région Noreste País Vasco Bizkaia Type d’activité Research Organisations Contact administratif Francisco Garcia (Mr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée TEKNOLOGIAN TUTKIMUSKESKUS VTT Finlande Contribution de l’UE Aucune donnée Adresse TEKNIIKANTIE 4 A 02044 VTT ESPOO Voir sur la carte Type d’activité Research Organisations Contact administratif Päivi Heimala (Ms.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée STICHTING WAGENINGEN RESEARCH Pays-Bas Contribution de l’UE Aucune donnée Adresse DROEVENDAALSESTEEG 4 6708 PB Wageningen Voir sur la carte Région Oost-Nederland Gelderland Veluwe Type d’activité Research Organisations Contact administratif Ruben Van Deursen (Mr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée
