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SEcreted Membrane vesicles: role in the therapeutic plasticity of neural StEM cells

Objective

Compelling evidence exists that somatic stem cell-based therapies protect the central nervous system from chronic inflammation-driven degeneration, such as that occurring in experimental autoimmune encephalomyelitis and stroke. It was first assumed that stem cells directly replace lost/damaged cells, but it has now become clear that they are able to protect the damaged nervous system through mechanisms other than cell replacement. In immune-mediated experimental demyelination and stroke we have shown that transplanted NPCs possess a constitutive and inducible ability to mediate efficient bystander myelin repair and axonal rescue. Yet, a comprehensive understanding of the mechanisms by which NPCs exert their therapeutic impact is lacking. We envisage that major NPC functions would result from highly sophisticated horizontal communication and we attribute a key role to the transfer of secreted membrane vesicles (MVs) from NPCs to neighbouring cells. Here we will focus on defining whether this form of communication exists for NPCs, and on elucidating its molecular signature and therapeutic relevance. We will investigate the MV small RNAome using next generation deep-sequencing, computational analysis and bioinformatics tools; and demonstrate that ncRNAs from NPCs are able to affect gene expression in neighbouring cells. Functional readouts of candidate ncRNAs will be provided by semi-quantitative RT-PCR and cutting-edge molecular and cellular biology tools [eg., miRNA-regulated LV vectors; identification of ncRNA targets by quantitative proteomics] on recipient cells. Then, we will demonstrate the biological impact of the transfer of individual small ncRNAs in vitro and in vivo in rodents with experimental neurological diseases. The true innovation of this project relies in its unique peculiarity to look into an innate mechanism with the visionary focus of translating the knowledge of basal stem cell functions into innovative high clinical impact therapeutics.

Field of science

  • /natural sciences/biological sciences/cell biology
  • /medical and health sciences/medical biotechnology/cells technologies/stem cells

Call for proposal

ERC-2010-StG_20091118
See other projects for this call

Funding Scheme

ERC-SG - ERC Starting Grant

Host institution

THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE
Address
Trinity Lane The Old Schools
CB2 1TN Cambridge
United Kingdom
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 1 410 932,40
Principal investigator
Stefano Pluchino (Dr.)
Administrative Contact
Renata Schaeffer (Ms.)

Beneficiaries (2)

THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE
United Kingdom
EU contribution
€ 1 410 932,40
Address
Trinity Lane The Old Schools
CB2 1TN Cambridge
Activity type
Higher or Secondary Education Establishments
Principal investigator
Stefano Pluchino (Dr.)
Administrative Contact
Renata Schaeffer (Ms.)
EUROPEAN MOLECULAR BIOLOGY LABORATORY
Germany
EU contribution
€ 89 067,60
Address
Meyerhofstrasse 1
69117 Heidelberg
Activity type
Research Organisations
Administrative Contact
Ratcliff Tom (Mr.)