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Novel Biocatalysts for the Production of Glycosides

Objective

This project aims to develop novel biocatalysts for the production of glycosides (NOVOSIDES). Glycosylated compounds have a wide range of applications, but very few enzymes are able to glycosylate small organic molecules cost-efficiently at the industrial scale. Therefore, glycosylation reactions catalysed by transglycosidases, glycoside phosphorylases and glycoside hydrolases will be explored in more detail. These enzymes catalyze the transfer a glycosyl group from a cheap and readily available donor substrate to a variety of acceptors. To exploit their full potential, the enzymes’ specificity and stability against high temperatures and the presence of organic co-solvents will be optimised by means of directed evolution.

A large and diverse collection of enzymes will first be established by screening in natural environments and by the mining of public (meta)genome databases. The enzyme collection will then be screened for activity on a variety of representative acceptors from different chemical classes. This will allow the identification of the most promising enzymes for optimisation through semi-rational and random mutagenesis. The high-throughput screening of natural and variant enzymes will be performed with newly developed fluorescent probes, that allow fast and accurate measurements of carbohydrate-active enzymes in a direct and non-destructive assay.

To achieve these ambitious goals, a complementary consortium of academia and industry has been formed that covers the whole range of required expertises. The economical potential of our technology will be demonstrated by the development and scale-up of selected glycosylation reactions at pilot-plant facilities. The produced glycosides will be actively marketed to potential end-users to promote the valorisation of the project’s results and to initiate future collaborations on novel target compounds.

Field of science

  • /natural sciences/computer and information sciences/databases
  • /natural sciences/biological sciences/genetics and heredity/genome
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins/enzymes

Call for proposal

FP7-KBBE-2010-4
See other projects for this call

Funding Scheme

CP-FP - Small or medium-scale focused research project

Coordinator

UNIVERSITEIT GENT
Address
Sint Pietersnieuwstraat 25
9000 Gent
Belgium
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 787 550
Administrative Contact
Wim Soetaert (Prof.)

Participants (5)

MIKROBIOLOGICKY USTAV AV CR V.V.I
Czechia
EU contribution
€ 472 600
Address
Videnska 1083
142 00 Praha 4
Activity type
Research Organisations
Administrative Contact
Vladimir Kren (Mr.)
RIJKSUNIVERSITEIT GRONINGEN
Netherlands
EU contribution
€ 521 000
Address
Broerstraat 5
9712CP Groningen
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Jan Poutsma (Mr.)
FRIEDRICH-SCHILLER-UNIVERSITAT JENA
Germany
EU contribution
€ 378 880
Address
Furstengraben 1
07743 Jena
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Alexander Schiller (Prof.)
BIO BASE EUROPE PILOT PLANT VZW
Belgium
EU contribution
€ 372 000
Address
Rodenhuizekaai 1
9042 Desteldonk Gent
Activity type
Other
Administrative Contact
Sofia Vindevogel (Ms.)
Carbosynth Limited
United Kingdom
EU contribution
€ 152 800
Address
Old Station Business Park 8 And 9
RG206NE Compton
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Vanessa Eastwick-Field (Dr.)