Objective Technologies for T cell visualisation and T cell depletion/enrichment are steadily making their way into the clinic and developments seem dictated by availability and reliability of the peptide-Major Histocompatibility Complex (pMHC) reagents used. A bottleneck has always been the laborious process of expression, refolding and purification of individual pMHCs. Recently, conditional MHC technology, pioneered in the host laboratory, solved this problem in part by enabling high-throughput loading of one MHC allele with a wide panel of peptide ligands. However, the rapid, parallel generation of a variety of MHC alleles is still a major hurdle. Therefore, proposed research aims at the total chemical synthesis of conditional MHC platforms, to make high-throughput generation of different MHC alleles reality. Recent advances in solid phase peptide synthesis (SPPS) have now enabled the generation of long polypeptides and even small proteins. This proposal aims to use SPPS rather than biological expression systems for total protein synthesis. The major advantage of using chemical techniques for protein synthesis is (1) that it allows the facile introduction of (non-natural) amino acids with functionalities that stabilise the protein by enhanced H-bonding, intrastrand crosslinking or intramolecular cross-linking (cyclisation) and that enable immobilisation or conjugation, (2) that protein yields are higher, and (3) that it simplifies the controlled generation of GMP grade proteins for biomedical use.The outcomes of this project will benefit the development of adoptive T cell therapy and will enable epitope scans through entire genomes of emerging pathogens (e.g. pandemic H1N1 Influenza), thus contributing to vaccine development. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health scienceshealth sciencesinfectious diseasesRNA virusesinfluenzanatural scienceschemical sciencesorganic chemistryaminesnatural sciencesbiological sciencesgeneticsgenomesmedical and health sciencesmedical biotechnologycells technologies Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) FP7-PEOPLE-2009-IEF - Marie Curie Action: "Intra-European Fellowships for Career Development" Call for proposal FP7-PEOPLE-2009-IEF See other projects for this call Funding Scheme MC-IEF - Intra-European Fellowships (IEF) Coordinator STICHTING HET NEDERLANDS KANKER INSTITUUT-ANTONI VAN LEEUWENHOEK ZIEKENHUIS EU contribution € 161 748,80 Address PLESMANLAAN 121 1066 CX Amsterdam Netherlands See on map Region West-Nederland Noord-Holland Groot-Amsterdam Activity type Research Organisations Administrative Contact Henri Van Luenen (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data
