Objectif This proposal aims at the development of novel nanostructured materials based on crystalline assemblies of anisotropic plasmonic (gold/silver) nanoparticles, to be used for the surface enhanced Raman scattering (SERS) detection of quorum sensing (QS) signaling molecules, and to the demonstration of applications of such materials to monitor population kinetics in bacterial colonies and the determination of the interaction mechanisms between mixed colonies and their manipulation through external parameters. This will involve a first stage related to the careful design of the most appropriate nanoparticle morphology and composition, as well as an understanding of their specific assembly processes (both on substrates and in solution), so that the collective plasmonic response will be optimized towards the enhancement of the Raman signal of the probe molecular codes. Coating of the nanoparticle supercrystals with a mesoporous layer will be required to protect them against contact with bacteria and cells, while permitting contact with the QS signaling molecules. Ultimately, when the sensing system has been optimized and its performance demonstrated for monitoring of QS signals and colony growth, two final and important goals will be pursued. First, the interaction between mixed colonies (bacteria-bacteria and bacteria-eukaryotic cell) will be monitored in order to get information about synergic or antagonist (toxicity) QS mechanisms during the growth and proliferation of different bacteria and interspecies. This goal will permit the design of in vitro experiments where a bacterial strain may be manipulated by means of external introduction of the appropriate QS signaling molecules. Finally, the major challenge will be the practical demonstration of the ability of these new materials in this particular configuration for understanding and manipulating the growth and communication of different types of prokaryotic and peukaryotic cells. Champ scientifique natural sciencesbiological sciencesmicrobiologybacteriologynatural scienceschemical sciencesinorganic chemistrytransition metalsengineering and technologymaterials engineeringcoating and filmsengineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsengineering and technologynanotechnologynano-materials Programme(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) ERC-AG-PE5 - ERC Advanced Grant - Materials and Synthesis Appel à propositions ERC-2010-AdG_20100224 Voir d’autres projets de cet appel Régime de financement ERC-AG - ERC Advanced Grant Institution d’accueil ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES- CIC biomaGUNE Contribution de l’UE € 1 376 133,61 Adresse PASEO MIRAMON 182, PARQUE TECNOLOGICO DE SAN SEBASTIAN EDIFICIO EMPRESARIAL C 20009 San Sebastian Espagne Voir sur la carte Région Noreste País Vasco Gipuzkoa Type d’activité Research Organisations Chercheur principal Luis Manuel Liz Marzán (Prof.) Contact administratif Alfonso Egaña (Mr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée Bénéficiaires (2) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES- CIC biomaGUNE Espagne Contribution de l’UE € 1 376 133,61 Adresse PASEO MIRAMON 182, PARQUE TECNOLOGICO DE SAN SEBASTIAN EDIFICIO EMPRESARIAL C 20009 San Sebastian Voir sur la carte Région Noreste País Vasco Gipuzkoa Type d’activité Research Organisations Chercheur principal Luis Manuel Liz Marzán (Prof.) Contact administratif Alfonso Egaña (Mr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée UNIVERSIDAD DE VIGO Espagne Contribution de l’UE € 871 496,30 Adresse LG CAMPUS LAGOAS MARCOSENDE 36310 Vigo Pontevedra Voir sur la carte Région Noroeste Galicia Pontevedra Type d’activité Higher or Secondary Education Establishments Contact administratif Anxo Moreira González (Mr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée