The role of small RNAs in remyelination

Projektinformationen

sRNAs remyelination

ID Finanzhilfevereinbarung: 275303

Abgeschlossenes Projekt

Startdatum 1 März 2012

Enddatum 28 Februar 2014

Finanziert unter

• Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Gesamtkosten € 179 101,60

EU-Beitrag € 179 101,60

Koordiniert durch

EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
Switzerland

Ziel

In diseases of the central nervous system (CNS) such as Multiple Sclerosis (MS), myelinating cells are the principal affected cells. Following pathological loss of myelin, remyelination by oligodendrocyte precursors (OPC) occurs but becomes increasingly incomplete and eventually fails in the majority of lesions. Understanding the molecular basis of myelination and remyelination and the regulation of the cells that achieve myelination and remyelination is primordial for both fundamental and clinical research.

The host lab and others have shown, mainly using conditional transgenic approaches in mice, that Dicer, an enzyme responsible for the maturation of small RNAs, including miRNAs, is essential for correct development of myelinating cells and their interactions with neurons. However, the functional role of small RNAs in adult remyelination has not been explored. Thus, the ETH Fellow will examine the importance of Dicer and DGCR8 (the latter is required specifically for miRNA maturation) during remyelination after injury. To achieve this goal, we will use conditional floxed alleles in the mouse that target these two genes. In combination with a well characterized tamoxifen-inducible PDGFRα-CreERT2 allele, we will eliminate DGCR8 and Dicer specifically in adult oligodendrocytes precursors cells followed by spinal cord injection of lysolecithin, a well-controlled demyelination/remyelination model. In parallel and in collaboration with other lab members, the ETH fellow will perform complementary neuroprotection experiments using a suitable neuron-specific inducible Cre recombinase line combined with the Dicer or the DGCR8 conditional lines. The work is embedded firmly in ongoing work in the host lab examining the functional role of Dicer and DGCR8 in glial development.

Wissenschaftliches Gebiet

• natural sciences › biological sciences › neurobiology
• medical and health sciences › basic medicine › neurology › multiple sclerosis
• natural sciences › biological sciences › genetics › RNA
• natural sciences › biological sciences › biochemistry › biomolecules › proteins › enzymes

Programm/Programme

• FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Thema/Themen

• FP7-PEOPLE-2010-IIF - Marie Curie Action: "International Incoming Fellowships"

Aufforderung zur Vorschlagseinreichung

FP7-PEOPLE-2010-IIF
Andere Projekte für diesen Aufruf anzeigen

Finanzierungsplan

MC-IIF - International Incoming Fellowships (IIF)

Koordinator

EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH

Kontakt Verwaltung

Ueli Suter (Prof.)

• EU-Beitrag: € 179 101,60
• Adresse: Raemistrasse 101 ; 8092 Zuerich ; Schweiz
• Region: Schweiz/Suisse/Svizzera › Zürich › Zürich
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: Keine Daten