Cel As the major target for therapy, Reverse Transcriptase (RT), and in particular the RT from the HIV virus, has been the subject of extensive research. However, despite an ever-increasing amount of information from traditional “bulk” studies, the detailed mechanisms of polymerization are still not completely understood. This is due, partially, to the stochastic nature of individual enzymes, which makes it impossible to follow more than one or two individual steps of polymerization using ensemble methods. I propose here to conduct a single-molecule, mechanistic study of HIV RT, by developing a high-resolution optical-tweezers, capable of resolving the individual polymerization and translocation steps of RT at a broad range of forces and nucleotides concentrations. Characterizing the dwell-time distribution at different experimental condition will allow us to study the complete mechano-chemical cycle of RT, its regulation by the template’s backbone-composition, structure and sequence, its interaction with additional viral and host factors, the molecular basis of inhibition by known inhibitors and the mechanism of resistance acquired through different mutations. Dziedzina nauki natural sciencesbiological sciencesmicrobiologyvirologymedical and health scienceshealth sciencesinfectious diseasesRNA virusesHIVnatural sciencesbiological sciencesgeneticsnucleotidesnatural sciencesbiological sciencesbiophysicsnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Program(-y) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Temat(-y) FP7-PEOPLE-2011-CIG - Marie-Curie Action: "Career Integration Grants" Zaproszenie do składania wniosków FP7-PEOPLE-2011-CIG Zobacz inne projekty w ramach tego zaproszenia System finansowania MC-CIG - Support for training and career development of researcher (CIG) Koordynator TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY Wkład UE € 100 000,00 Adres SENATE BUILDING TECHNION CITY 32000 Haifa Izrael Zobacz na mapie Rodzaj działalności Higher or Secondary Education Establishments Kontakt administracyjny Mark Davison (Mr.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych
