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Content archived on 2024-06-18

Hydrogel-Phage Composite Materials and Droplet Microfluidics

Objective

Virus particles are increasingly used as building blocks for composite materials. Surface modification of these biological nanoparticles can be accomplished by genetic engineering and offers the possibility to combine directed evolution of molecular function with the assembly of more complex materials and devices. Virus display systems, especially phage display, have been extensively used for evolving peptides and proteins capable of specific binding to a diverse range of biomacromolecules and inorganic materials. Recently life science approaches emerged using virus particles as substitute antibodies in diagnostic tools. To harvest the full potential in connecting virus display technology with materials, smart fluid handling approaches must be applied allowing to process high numbers of samples. This project is about combining droplet microfluidics, a technology dealing with monodisperse water in oil emulsion droplets, with phage display. The emulsion droplets represent isolated vessels with volumes in the pico- to nanoliter range and can be split, merged, incubated and sorted at high frequency. In addition, hydrogel matrixes will be applied. They serve as scaffold for phage immobilization and allow to transfer the sample from emulsion to an aqueous phase while retaining compartimentalization. The resulting gel beads will be designed as such that proteins can diffuse freely while the phages are immobilized in the matrix. The concept will be applied for directed evolution of enzymes and the development of novel diagnostic tools.

Fields of science (EuroSciVoc)

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP7-PEOPLE-2009-IEF
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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MC-IEF - Intra-European Fellowships (IEF)

Coordinator

THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE
EU contribution
€ 180 603,20
Address
TRINITY LANE THE OLD SCHOOLS
CB2 1TN CAMBRIDGE
United Kingdom

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Region
East of England East Anglia Cambridgeshire CC
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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