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Content archived on 2024-05-27

Molecular mechanisms leading to genome-wide DNA demethylation during epigenetic reprogramming

Objective

Epigenetic reprogramming is a developmental process involving both genome-wide DNA demethylation and chromatin remodelling. Under physiological conditions it occurs at distinct stages of normal embryonic development. However, under in vitro conditions induction of this process can restore pluripotency in somatic cells, and in aberrant situations a similar dedifferentiation process can initiate carcinogenesis. While the kinetics of the reprogramming that occurs in the course of normal development has been well documented, initial steps inducing this process and how DNA methylation marks are removed in vivo remain elusive. The involvement of DNA repair mechanisms in DNA demethylation during epigenetic reprogramming has been recently suggested. The presented project proposes to investigate the upstream events initiating epigenetic reprogramming by using both in vivo and in vitro approaches. We will focus on recently discovered candidates involved in 5-methylcytosine hydroxylation and will investigate a possible involvement of this modification of 5-methylcytosine in the epigenetic reprogramming during mouse development. We will also examine the potential repair enzymes able to remove this epigenetic mark in order to highlight other protein partners involved in this process. Data obtained on this pathway will not only enrich our understanding towards developmental reprogramming, but also enhance our ability to manipulate cell fate in vitro.

Fields of science (EuroSciVoc)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP7-PEOPLE-2010-IEF
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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MC-IEF - Intra-European Fellowships (IEF)

Coordinator

IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE
EU contribution
€ 199 549,60
Address
SOUTH KENSINGTON CAMPUS EXHIBITION ROAD
SW7 2AZ London
United Kingdom

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Region
London Inner London — West Westminster
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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