Results 1 - 10 of 2277
[REPORT SUMMARY] Genotyping of isolates using (GTG) 5 repetitive PCR
Molecular Characterization of aquaculture-associated bacteria by (GTG)5 repetitive PCR
Epidemiological typing of bacteria or pathogens has become more and more important in public health control. PCR is a powerful technique that has revolutionized the molecular biology. Repet...
Programme: FP5-INCO 2
Record Number: 42595
Last updated on: 2006-11-28
[REPORT SUMMARY] Plasma enhanced deposition of SiO2-like innovative coatings as protective barrier layers for bronze archaeological artefacts
New corrosion resistant coatings for the protection of bronze archaeological artefacts, by using clean and environmentally friendly processes, have been developed and optimised. The coatings deposition has been performed by PECVD technique (plasma enhanced chemical vapour depo...
Programme: FP5-INCO 2
Record Number: 41806
Last updated on: 2008-01-14
[REPORT SUMMARY] Advantages verification of seawater electrolysis technique
The formation of an adequate physical-mathematical model of sea water electrolysis requires basis in theory together with some experimental investigations. The experimental investigations of sea water electrolysis resulting in hydrogen as its main production target and some as...
Programme: FP5-INCO 2
Record Number: 42827
Last updated on: 2006-11-28
[REPORT SUMMARY] Plate-based conjugation assay for enterobacterial isolates
A plate-based conjugation assay was optimised using a known donor isolate, E. coli RC85 (chloramphenicol resistant) and a known recipient E. coli isolate HB101 (streptomycin resistant).
Chloramphenicol resistant project isolates shown to be susceptible (> 6 mm zone size) to s...
Programme: FP5-INCO 2
Record Number: 42624
Last updated on: 2006-11-28
[REPORT SUMMARY] Central collection of chloramphenicol-resistant heterotrophs from aquaculture environments
A central backup collection of all isolates collected in this project was established that is accessible to all partners of the project. The principle of storage is based on cryopreservation using the SOP-ASIARESIST-PRES document. The final strain collection consists of 724 ch...
Programme: FP5-INCO 2
Record Number: 42601
Last updated on: 2006-11-28
A Standard Operating Procedure (SOP) was defined for the antibiotic susceptibility testing of project isolates using the disc diffusion (DD) method (SOP-ASIARESIST-ABR). The final version of the SOP was obtained following intensive discussions among partners and was made avail...
Programme: FP5-INCO 2
Record Number: 42598
Last updated on: 2006-11-28
[REPORT SUMMARY] (GTG)5- PCR mediated typing of aquaculture-associated isolates
A repetitive DNA element PCR fingerprinting (rep-PCR) protocol was developed for the strain typing of aquaculture-associated bacterial isolates (n= 650). A simple boiled extract of broth cultures acted as a DNA template for the PCR.
The (GTG)5 primer was employed in combinati...
Programme: FP5-INCO 2
Record Number: 42619
Last updated on: 2006-11-28
Isolation of chloramphenicol resistant bacteria from aquaculture farms in Malaysia.
Antimicrobial agents have been widely used in aquaculture worldwide to treat infections caused by a variety of bacterial pathogens. This study undertaken is the evidence of the occurrence of a...
Programme: FP5-INCO 2
Record Number: 42593
Last updated on: 2006-11-28
[REPORT SUMMARY] Macrorestriction analysis of aquaculture-associated isolates
Pulsed-field gel electrophoresis of macrorestriction fragments was applied to confirm the clonal relationships of aquaculture-associated genera previously inferred by (GTG)5-PCR typing. Cell lysis, restriction enzymes and electrophoresis running conditions were optimised for e...
Programme: FP5-INCO 2
Record Number: 42620
Last updated on: 2006-11-28
[REPORT SUMMARY] Characterization and conjugation of chloramphenicol resistant gene
Resistance gene detection and horizontal transfer.
To increase the rapidity and accuracy of resistance testing, the use of PCR (a genotypic approach and DNA-based assay) for the detection of bacterial resistance was developed. This novel approach is a true revolution because ...
Programme: FP5-INCO 2
Record Number: 42596
Last updated on: 2006-11-28
List retrieved on: 2018-04-26
