Community Research and Development Information Service - CORDIS


Clean-up HPLC method for the determination of ochratoxin A in baby food at a possible future European regulatory limit. The test portion of the sample was extracted in a blender with tert-butyl methyl ether after addition of 0.5 mol/L phosphoric acid/2 mol/L sodium chloride solution. The sample extract was centrifuged and re-dissolved in a mixture of phosphate buffered saline solution and methanol. After removal of lypophilic substances with hexane, the extract was applied to an immunoaffinity column containing antibodies specific to ochratoxin A. The column was awshed with water to remove the interfering compounds while the purified ochratoxin A was eluted with methanol. The separation and determination of ochratoxin A was performed by reverse-phase high performance liquid chromatography and detected by fluorescence after post column dramatization with ammonia. The baby food samples, both spiked and naturally contaminated with ochratoxin A, were sent to 13 laboratories in 8 different European countries. Test portions of samples were spiked at a level of 0.085 ng/g ochratoxin A. The average recovery for the spiked blank baby food was 109%. Based on results for spiked samples as well as naturally contaminated samples the relative standard deviation for repeatability (RSD(r)) ranged from 18% - 36%. The relative standard deviation for reproducibility (RSD(R)) ranged from 29% - 63%. The method showed acceptable within-laboratory and between-laboratory precision for this matrix, as evidenced by HORRAT ratios, at the target levels of determination for ochratoxin A.

Additional information

Authors: BURDASPAL P A, Centro Nacional de Alimentación, Madrid (ES);MA LEGARDA T, Centro Nacional de Alimentación, Madrid (ES)
Bibliographic Reference: EUR 19718 EN (2001), pp. 35. Euro: Free of charge
Availability: Available from Documentation service DG Research. Fax: +32-2-2958220
ISBN: ISBN 92-894-0499-X
Follow us on: RSS Facebook Twitter YouTube Managed by the EU Publications Office Top