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Final report on Contract No. SMT4-CT96-2047 (Part I).

The report describes research performed on problems in trichothecene analysis with gas chromatography and a comparative interlaboratory study on method performance. Several method problems were identified during the first intercomparison study in the project and the following discussions. They were a) higher trichothecene response in presence of matrix, b) non-linear calibration curves, c) drifting response for the trichothecenes, d) carry-over or memory effects from previous sample, e) matrix interference. These problems were studied in two laboratories with different methods and type of equipment. The results from these studies are presented. Based on these results recommendations were given to participating laboratories how to reduce or eliminate the identified method problems. The laboratories were given six months for implementation of recommendations in their methods before the start of the joint method performance study.

Most laboratories had still a matrix effect on the trichothecene response in the performance study. Laboratories using mass detection got mainly exponential calibration curves and had a high variation in the injection repeatability. The recovery of trichothecenes were in many cases higher than 110% and/or with a variation of more than 20%, when external calibration without matrix was used. The use of matrix assisted calibration or internal GC-standard with or without matrix assistance lowered the matrix effect and the variation in the trichothecene analysis with gas chromatography. These approaches are, however, questionable for methods used for certification of reference materials.

Additional information

Authors: PETTERSSON H, Swedish University of Agricultural Sciences, Department of Animal Nutrition and Management, Uppsala (SE);LANGSETH W, National Veterinary Institute, Oslo (NO)
Bibliographic Reference: European Commission, DG Research, 2002. EUR 20285/1 EN, pp.82. (BCR Information series) Free of charge
Availability: EUR-OP reference: KI-NA-20285-EN-C Available from: Documentation Service of DG Research Fax: +32 2 295 8220 E-mail:
ISBN: ISBN: 92-894-3449-X
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