Community Research and Development Information Service - CORDIS



Project ID: 309433
Funded under: FP7-IDEAS-ERC
Country: Spain

Mid-Term Report Summary - EPIPLURIRETRO (Epigenetic control and impact of mammalian retrotransposons in pluripotent genomes)

New LINE-1 retrotransposition events in humans occur during early embryogenesis (i.e., pluripotent genome). Thus, it stand to reason that restriction mechanisms must exit in those cells to avoid high rates of mutagenesis in our germ line genome. Data from our laboratory and others have demonstrated that human LINE-1 elements are controlled epigenetically with regards to: i) the fate of L1 retrotransposition and ii) the control of L1 expression. However, we know little about these mechanisms and what the impact of new retrotransposition events is on pluripotent cells. EpiPluriRetro aims to understand epigenetic mechanisms operating in our pluripotent genome that regulate retrotransposition and how new retrotransposition events can alter the epigenome of cells. The main objective of EpiPluriRetro is to identify epigenetic mechanisms that operate in our pluripotent genome that: i) act to control the fate of new L1 retrotransposition events (initiation and maintenance) and ii) control the expression of mammalian retrotransposons. In addition, we aim to systematically determine the “epimutagenic” impact associated with new L1 mobilization events in pluripotent genomes, and the effect of L1 insertions within human genes.

So far, research within Epipluriretro has demonstrated that new L1 insertions in genes of pluripotent cells can alter gene expression by a myriad of mechanism. Similarly, we are currently deciphering the degree of epigenetics alterations produced by new retrotransposition events in pluripotent cells.

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