Forschungs- & Entwicklungsinformationsdienst der Gemeinschaft - CORDIS


LT-NRBS Berichtzusammenfassung

Project ID: 311529
Gefördert unter: FP7-IDEAS-ERC
Land: Spain

Mid-Term Report Summary - LT-NRBS (Lab-in-a-tube and Nanorobotic biosensors)

In this project, we proposed a new paradigm technology based on the shrinkage of “Lab-on-a-chip” to “Lab-in-a-tube” microsystems as well as the use of catalytic microbots as biotechnological tools. In order to carry out this project, we adapted the rolled-up technology stablished at the initial host institution for life science applications. Once we optimized the rolling-up of thin films (metal oxides and metals), we functionalized the interior for bio-relevant applications. The major highlights are summarized here.
Lab-in-a-tube: Up to date, most of the studies on cellular behaviours have been explored only on planar 2D patterns, which do not mimic the in vivo microenvironment of the cells. During the first part of the ERC Project, we used micropatterning and strain engineering to encapsulate single living mammalian cells into transparent tubular architectures consisting of three-dimensional (3D) rolled-up nano-membranes. We showed that spatial confinement of mitotic mammalian cells inside tubular architectures can perturb metaphase plate formation, delay mitotic progression, and cause chromosomal instability in both a transformed and nontransformed human cell line (Wang Xi, et al. NanoLetters 2014, 14, 4197).
Additionally, rolled-up transparent microtubes are shown to serve as cell culture scaffolds that exactly define the space available for single cell growth. Human U2OS osteosarcoma cells are confined within microtubes of different diameters and the effects of the cell deformation on the integrity of the DNA and cell survival are studied (B. Koch et al., Adv. Healthcare Mater. 2014, 11, 1753).
We can even engineer porous cylindrical scaffolds with patterns of holes, which are capable of mimicking microvasculatures. The porous tubes, which can be made from diverse materials for differential functionalization, are biocompatible and can be modified to be biodegradable in the culture medium. Endothelial cells (ECs) as well as astrocytes were cultured on these scaffolds. Eventually, this material could also possibly be implanted in vivo due to its biodegradability (R. Arayanarakool et al., Lab Chip 2015, 15, 2981).
The rolled-up microtubes not only serve as 3D micro-reactors for live cell studies but also as on-chip integrated sensors. We reported the detection of analytes by rolled-up compact sensing devices. We engineered ultracompact three-dimensional tubular structures integrating Au-based electrodes as impedimetric microsensors for the in-flow determination of mono- and divalent ionic species and HeLa cells. The microsensors show an improved performance compared to conventional planar conductivity detection systems integrated in microfluidic platforms and the capability to detect single HeLa cells in flowing phosphate buffered saline. These highly integrated conductivity tubular sensors open new possibilities for lab-in-a-tube devices for analytical and bioanalytical sensing and bioelectronics. (Martínez-Cisneros, et al. Nano Lett., 2014, 14 (4), 2219)

Catalytic micro- nanomotors: The rolled-up nanotechnology enables the fabrication of tubes with a large variety of materials including metals. When the tubes can be released “off-chip” and be suspended in solution where they can navigate as microjets by bubble propulsion mechanism.
These tiny machines have demonstrated interesting capabilities to transport cargoes to specific targets in an accurate manner and potential applications in biosensing, biochemical and environmental remediation. This big challenge inspires us to seek for other biocompatible and clean sources of motion to expand the field of nanomotors to real biomedical and environmental applications. The use of nanomotors as selective biosensors is hence of ground-breaking nature.
Sánchez’s group has been consolidated as one of the leading groups in catalytic nanorobotics/motors.
These miniaturized intelligent devices do not require external energy sources. Instead, they locally convert chemical energy into mechanical energy by means of catalytic reactions. Sánchez has shown several proof-of-concepts in biomedicine, sensing, microfluidics and environmental science. This is a hot topic that attracts a lot of attention from the scientific community and the media. The group reported pioneering works on different sub-topics, such as the discovery of “chemotaxis” for spherical and tubular microrobots (Baraban et al. Angew.Chem.Int.Edit. 2013, 52, 5552) and the first microrobot cleaning contaminated water (Soler et al. ACS Nano 2013, 7, 9611). In topics closely related to the ERC project, we reported the first hybrid Micro-bio-robot from sperm cells and magnetic microtubes (Magdanz et al. Adv. Mat. 2013, 25, 6581)
This fast development gave the Grantee about 25 publications in this topic in high quality journals and more than 10 covers. His strong impact in the field of nanomotors at his young age has attracted the attention from editors from Angew.Chem., inviting him to write a comprehensive review on the topic (Sanchez et al. Angew.Chem.Int.Edit. 2015, 54, 1414).

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