Community Research and Development Information Service - CORDIS


LUNAR.CLOCK Report Summary

Project ID: 337011
Funded under: FP7-IDEAS-ERC
Country: Austria

Mid-Term Report Summary - LUNAR.CLOCK (Molecular neurobiology of a moonlight entrained circalunar clock)

Over the past 30 months we successfully finished the establishment of TALEN-mediated genome mutagenesis in Platynereis dumerilii (doi: 10.1016/j.cub.2014.06.032, doi: 10.1534/genetics.112.148254, doi: 10.1534/genetics.113.161091). We have determined the spectral sensitivity of several photoreceptors and generated mutants, which we currently analyze with a range of different molecular and behavioral assays. In order to allow higher through-put, as well as to minimize the variation introduced by the different experimenters, we successfully automatized the locomotor behavioral analyses for the bristle worm Platynereis dumerilii (collaboration with Prof. Andrew Straw and further developments with loopbio GmbH). Besides the investigation of the worm’s different photoreceptors, we also investigate how much the monthly (circalunar) clock impacts on the level of brain transcripts. An initial screen with about 500 pre-selected genes and the verification and expression analysis of circalunar-regulated candidate genes is close to completion (Nanostring). We have complemented this ‘biased’ screen with additional ‘unbiased’ transcriptomic and proteomic screens, which are currently amidst analysis.
Finally, in addition to our work on the circadian and circalunar clocks in Platynereis, we have also investigated circadian and circalunar timing variations in the marine midge Clunio marinus. We identified a molecular mechanisms that could be of relevance also to human chronotypes. (This work is currently in press in Nature.)

Reported by

Follow us on: RSS Facebook Twitter YouTube Managed by the EU Publications Office Top