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Oral delivery of chemotherapeutics in marine larviculture of fish

The objectives of this work were to examine further the potential of bioencapsulation of chemotherapeutics in the live food stuffs Brachionus and Artemia for the prevention and/or treatment of disease in larval stages of selected species (seabass, seabream) and to attempt pre-industrial application of the methodology.

Two novel products were produced, namely liposomes loaded with oxytetracycline, and a medicated HUFAl emulsion containing quinolone antibacterials.

New high performance liquid chromatography (HPLC) methods were developed for the quantification of the therapeutics in Artemia nauplii, fed either with the liposomes or with the medicated emulsion. Although liposomes were taken up by Artemia, they proved rather expensive for use on a large scale. However, the commercial emulsion in which quinolones were incorporated, was successfully uptaken by the nauplii and proved quite efficient.

Challenge tests performed on seabass larvae exposed to Vibrio showed that when fish are fed with Artemia enriched with the quinolone emulsion, mortalities are greatly reduced. The bioencapsulation treatments proved more effective, economical and friendly to the environment than the already used immersion methods, where the therapeutics are added in the water column.

Reported by

Aristotle University of Thessaloniki
School of Biology University Campus
54006 Thessaloniki