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ERC

SINGLESITE Report Summary

Project ID: 294509
Funded under: FP7-IDEAS-ERC
Country: France

Final Report Summary - SINGLESITE (Pre- and postsynaptic signaling at single synaptic contacts)

This project established a series of new methods for the electrophysiological recording of vesicular release in small synapses comprising a single presynaptic active zone and a single postsynaptic density ('simple synapses'). We found that the output of simple synapses is limited, suggesting the presence of several docking/release sites per simple synapse. Docking/release site numbers vary among simple synapses. Docking/release site numbers are very similar to the numbers of clusters of voltage-gated calcium channels in the active zone, suggesting a close association of one docked synaptic vesicle with one cluster of calcium channels. The statistics of vesicular release at simple synapses are best explained by a sequential model called 2-step model. In this model, each vesicle associates first to a 'replacement site' and then to an associated docking site before being released. Docking site occupancy emerges as a key parameter governing synaptic function. Changes in docking site occupancy are in particular responsible for short term synaptic plasticity (synaptic facilitation and synaptic depression).

Reported by

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
France
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