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SOX10mutants Report Summary

Project ID: 661616
Funded under: H2020-EU.1.3.2.

Periodic Reporting for period 1 - SOX10mutants (Investigating genotype-phenotype correlations in SOX10 neurocristopathies)

Reporting period: 2015-07-01 to 2017-06-30

Summary of the context and overall objectives of the project

One of the critical events during the development of an embryo is the formation of a transient structure called the neural crest. Cells from the neural crest give rise to a wide range of cell types in the body, including cartilage and bone cells, pigment cells, nerve cells in the gut and nerve-supporting cells (called glia). Abnormalities in neural crest cells can give rise to developmental syndromes in humans, such as Waardenburg syndrome (WS) and Hirschsprung disease (HD). In HD, sections of the gut fail to develop nerve cells, which can be life-threatening for undiagnosed infants and for which the only treatment option currently is surgery. Patients with WS suffer a range of symptoms, including hearing loss and defects in pigmentation of the hair, eyes and skin. WS is a rare disease and thus little is known about its health and economic burdens, or about its prevalence. However, in a single study, pooled cohorts from the US, UK and South Africa described 274 individuals as WS sufferers and over 50 cases have been reported in Europe alone. WS is divided into 4 sub-types, WS types 1 to 4, which differ in symptoms, with WS types 1 and 2 being the most common. WS type 4 is particularly debilitating as it is associated with HD. While first described in the Dutch population, with an estimated incidence of 1 in 42 000 people, WS occurs in many racial groups and an incidence of 1/20 000 people was reported in Kenyan Africans. The various sub-types of WS have been associated with mutations in several genes, mainly transcription factors involved in neural crest cell specification and pigmentation such as PAX3, MITF and SOX10. Of particular interest to this study are the mutations in SOX10 associated with WS types 2 and 4, as well as with a severe neurological variant of WS type 4 called PCWH (Peripheral demyelinating neuropathy–Central dysmyelinating leukodystrophy–Waardenburg syndrome–Hirschsprung disease).

SOX10 plays a key role in the production, movement and fate of neural crest cells. The role played by SOX10 in neural crest cells stems from its ability to bind to the DNA and regulate expression of its target genes. Interestingly, a number of patients with WS have been shown to have subtle mutations in SOX10 within the DNA binding domain, a region of the protein that is important for its transcription factor activity. When these mutations were tested using cell culture (in vitro) methods, the results did not match well to the severity of the symptoms of the WS patient from which the mutation was isolated (we say there is no genotype-phenotype correlation). This is likely due to the fact that it is very difficult to reproduce the complex environment of the neural crest using cell culture experiments. It was therefore clear that new tools were needed to better understand the genotype-phenotype relationship in neural crest disorders using a living organism (an in vivo context).

The SOX10mutants project proposed to address this need by using a novel zebrafish rescue assay to investigate the effects of different mutations in the SOX10 gene on the development of cell types deriving from the neural crest, to test in vivo the genotype-phenotype correlations with WS. Importantly, sox10 gene expression and function is extremely similar between fish and mammals and the rescue assay was based on the use of a zebrafish line in which sox10 is mutated and which lacks melanocytes (pigment cells) and enteric neurons (nerve cells in the gut), similar to WS symptoms. The objectives of this study were, firstly, to determine if expressing the human mutant SOX10 proteins in the mutant zebrafish embryos was able to rescue the lack of melanocytes and enteric neurons. Secondly, we wished to determine in which cellular compartments the mutant proteins were located and thirdly, to explore the details of how the mutant proteins might act at a molecular level.

Our initial approach to in vivo testing worked well for melanocytes, but it proved d

Work performed from the beginning of the project to the end of the period covered by the report and main results achieved so far

The SOX10mutants project began with the researcher acquiring a personal license for zebrafish work, gaining familiarity with the model system and learning the experimental techniques necessary for the project. The initial rescue assay experiments required testing conditions to achieve reproducible results. While melanocyte rescue could be demonstrated with the human SOX10 proteins, we were initially unable to rescue enteric neurons, despite many modifications to the experimental conditions. We hypothesized that increasing the expression of the human SOX10 may allow rescue of enteric neurons and through significant change to the experimental parameters, we did indeed see partial rescue of enteric neurons. When the results for melanocyte and enteric neuron rescue of the various SOX10 mutants were compared to WS patient symptoms, there was a better genotype-phenotype correlation using the zebrafish assay than cell culture experiments. Furthermore, analysing expression of selected mutants for their cellular location in vivo showed that our results generally matched those previously reported from in vitro assays. Work from this project was presented at various forums, including oral presentations at the University of Bath and the University of Cape Town, to the general public at the University of Bath Science Fair and to students at two local schools in Bath. We have written a book chapter on the rescue assay technique and are also preparing a peer-reviewed paper for publication in the near future.

Progress beyond the state of the art and expected potential impact (including the socio-economic impact and the wider societal implications of the project so far)

The outcomes of the SOX10mutants project were significant and very promising. Our aim is to extend and expand this project by using the zebrafish model system to analyse selected WS associated SOX10 mutations in greater depth, again in collaboration with our Imagine Institute partners. This line of investigation has great potential for helping us understand how subtle mutations in the human SOX10 gene give rise to the different symptoms of the WS syndrome subtypes. This is information that is currently unavailable to patients suffering from the disorder and the results from this work are expected in the future to contribute to genetic counselling offered to WS patients. This work could also be the first step towards using zebrafish as a tool for screening therapeutic options that could alleviate the symptoms of this disorder.

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