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Entrapment of Hypoxic Cancer by Macrophages Loaded with HAP

Periodic Reporting for period 1 - McHAP (Entrapment of Hypoxic Cancer by Macrophages Loaded with HAP)

Reporting period: 2017-01-01 to 2018-06-30

TfR1 knock-down in THP-1 inhibited transferring uptake (upper) but did not affect Ft uptake (bottom
Cancer cell apoptosis due to co-culture with macrophages loaded with cyclophosphamide and chlorambuc
TfR1 knock-down inhibits in Ft uptake from medium in cancer cells (here an example of A549)
Transwell co-culture experiment showed that only direct cell-cell contact ensures TRAIN
TfR knock-down did not influence TRAIN in human cancer cell lines
Doxorubicin localization in cancer cells after administration in ferritin cage
The THP-1 macrophage ferritin uptake dependent on time and conc. (upper) and also temp (bottom)
TfR1 knock-down in THP-1 cells
Confirmation of results in cancer cell lines with high and low TRAIN efficacy by Real-time PCR
Co-localization of Ft (green) and red: EEA1, LAMP1 and lack of co-localisation with Rab11 and TF
Gene expression clustering in cancer cell lines with high and low TRAIN efficacy
TRAIN efficacy in various cancer cells (left) and its correlation with TfR and CD47 expression (dots
No effect on wortmannin on TRAIN efficacy(left) and inhibitory Cytochalasin D effect on TRAIN(right)
Cancer cell apoptosis due to co-culture with macrophages loaded with cyclophosphamide and chlorambuc

Project information

Grant agreement ID: 715048

Status

Ongoing project

  • Start date

    1 January 2017

  • End date

    31 December 2021

Funded under:

H2020-EU.1.1.

  • Overall budget:

    € 1 413 750

  • EU contribution

    € 1 413 750

Hosted by:

SZKOLA GLOWNA GOSPODARSTWA WIEJSKIEGO