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Evaluation of Bcl-2 over-expressing NS0 cell lines

The NS0 cell line over-expressing Bcl-2 was evaluated for its potential to act as a host cell line for expression of recombinant antibodies. The cell line was taken through the generic cell line construction process used at Lonza Biologics using a model antibody. Antibody accumulation and growth kinetics were evaluated at different stages in the process.

Finally, cell lines were evaluated in bioreactors using a scale-down model (10 L) of a process operated at 5000 L. No benefit was seen from using the Bcl-2 over-expressing cell lines: antibody concentrations were substantially lower than those seen with the control cell lines.

Reported by

Lonza Biologics
228 Bath Road
SL1 4DX, Slough
United Kingdom
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