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Bacillus vectors for epitope and localization tagging of proteins

A total of six integrational plasmid vectors were constructed for use in Bacillus subtilis. These vectors allow addition of two different kinds of tags to the C-terminal end of any protein encoded by the B. subtilis chromosome. In one case, an epitope tag is added (cMyc, HA, FLAG)which allows detection of proteins by commercially available antibodies.

This method can be used for affinity purification of proteins of interest and for their detection in Western blots. In the other case, a localisation tag is added to the proteins of interest (GFP, CFP, YFP) allowing their detection within one of the compartments of the B. subtilis cell. In addition, these tagged proteins can be detected in Western blots.

The results have been published (Kaltwasser et al., 2002, Appl. Environm. Microbiol. 68, 2624-2628).

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University of Bayreuth
Institute of Genetics, University of Bayreuth, University Str 30
95440 Bayreuth