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EXMAD Informe resumido

Project ID: HPRI-CT-1999-50015
Financiado con arreglo a: FP5-HUMAN POTENTIAL
País: Austria

Device for pressurisation of crystals with rare, expensive or toxic gases

An instrument to generate derivatives of protein crystals with gases, such as Xenon and Krypton, and to rapidly freeze-trap these crystals in order to prevent desorption of the bound gas has been constructed. The instrument was designed to be fully computer-controlled to allow easy operation and to permit reproducible gas-charging parameters (pressure and time of exposure, profile of pressure build up and release, etc).

Moreover, no pressurised rare-gas should be required, and the gas should be fully confined and recyclable in order to permit work with very expensive or very toxic gases. The instrument consists essentially of a piston to pressurize the gas up to 100bar. The protein crystal is picked-up from its cryo-protectant with a standard cryo-loop mounted in a special pin. This pin is attached to a pin holder, which snaps into the instrument. From that point on, operation is under computer control until the crystal is flash-cooled.

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Reported by

Karl-Franzens-Universitaet Graz
Heinrichstrasse 28
8010 Graz
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