Forschungs- & Entwicklungsinformationsdienst der Gemeinschaft - CORDIS

Method for identification of trichothecene and enniatin producing Fusarium species in cereals

The result is a set of PCR methods to detect Fusarium species that produce trichothecenes and/or enniatins.

Assays to the gene encoding trichodiene synthase provide generic detection of trichothecene producing species while assays to the gene encoding enniatin synthetase provide generic detection of enniatin producing species. Assays to the gene tri4, encoding the second step in trichothecene biosynthesis broadly differentiate between producers of type A and type B trichothecenes, the former being significantly more toxic to humans than the latter.

The result also includes assays to differentiate between isolates that produce nivalenol and those that produce deoxynivalenol (both type B trichothecenes). The former is significantly more toxic to humans than the latter. This set of assays provides a powerful tool to incorporate into crop disease management and risk assessment models for mycotoxin occurrence across Europe and elsewhere. The set of assays has been developed to permit highly sensitive detection of species able to produce the relevant toxins, within cereal plant tissues and other matrices.

The method will be published within peer-reviewed scientific journals. Licence agreements are foreseen with laboratories both within and outside Europe.

More information on the DETOX-FUNGI project can be found at:

Reported by

Norwich Research Park, Colney
United Kingdom
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