Servicio de Información Comunitario sobre Investigación y Desarrollo - CORDIS

Method for detection, quantitation and differentiation of ochratoxigenic Penicillia

A molecular PCR method for detection, quantification and differentiation of ochratoxigenic Penicillia has been developed. It differentiates between P. verrucosum and P. nordicum. Both species are able to produce ochratoxin and are morphologically very similar.

For differentiation two primer pairs are used giving rise to two specific fragments for P. nordicum, but only one fragment for P. verrucosum. Based on the specific fragment for P. nordicum a quantitative Real Time PCR system has been developed.

The system is very robust and it has been shown that it is functional in food matrices like wheat. It could be used to monitor the growth kinetics of P. nordicum in wheat.

More information on the DETOX-FUNGI project can be found at:

Reported by

Federal Research Centre for Nutrition
76131 Karlsruhe
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