Servizio Comunitario di Informazione in materia di Ricerca e Sviluppo - CORDIS

Acute phase protein in experimental models of porcine infectious diseases

A number of combinations of acute phase proteins (APP) were found to work well in detecting infectious/inflammatory processes, the most sensitive combinations in general being (Hp, apoA1, pigMAP) and (CRP, apoA1, pigMAP). These are as good as or only very slightly lower than four protein combination in all cases.

An acute phase index based on such a combination of APPs (for example (CRPxpigMAP) / ApoA1)), thus including a negative APP, would give an increased robustness of conclusions based on these measurements and would correct for possible dilution and evaporation artefacts.

It could be argued that the combination with Hp is less usable than the other one, as pre-infection levels of Hp vary considerably making it a more difficult task to determine a cut-off value for this protein as compared to the others.

It also seems worthwhile to consider some of the two-protein combinations (e.g. (Hp, pig MAP); (Hp, ApoA1) and (CRP, apoA1)), as these two-protein combinations perform almost as well as the three-protein combinations.

Since the situation may be different with real samples, all promising combinations of proteins should be tested on real herd derived blood samples before a final decision is made.

It furthermore remains to define operational, real-life cut-off values for the APPs to be tested in an acute phase index and for the index value based on these APPs.

Thus, in conclusion, the combinations of (Hp, apoA1, pigMAP) or (CRP, ApoA1, pigMAP) give the most sensitive detection of inflammation/infection, but two-protein combinations like (Hp, ApoA1) should also be considered, especially when a precise cut-off level for Hp is at hand.

The aim of an acute phase index based on such a combination of APPs will be a high specificity (few false positives). Such an index will enable us to detect with great certainty an ongoing infection/inflammatory situation, but will not enable us to declare an animal healthy in the absence of an elevated APP-level.

More information on the APP IN PIG PRODUCTIO -project can be found at:

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Reported by

Danish Institute for Food and Veterinary Research
Bulowsvej 27
1790 V Copenhague
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