Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

Method for sample preparation and HPLC analysis for quantification of phenols in asphalt

A method was developed subjecting the asphalt sample to a soxhlet extraction with CH2Cl2 (2mL solvent per g asphalt) during 4 hours. For purification of this extract, a liquid-liquid extraction method was developed transferring the phenols from the CH2Cl2 phase into an alkaline aqueous solution at pH 14. The recovery of this step in the presence of matrix was determined (100% for phenol, but only 34% for 1,3,5 trimethylphenol. The alkaline solution is neutralized, filtered and directly injected into HPLC. An isocratic reversed phase HPLC method using a special C18 column with polar modification was developed for an appropriate separation of the 10 selected phenols. Quantification of the phenols is carried out using individual external calibration. The use of a UV photodiode array detector enables peak identification. For the application to the asphalt samples, trace determination capability was required, since several phenols are present below 1ppm.

Reported by

Saarland University
Im Stadtwald, Campus der Universität, Bau 9.2
66123 Saarbrücken