Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

Species-specific probiotic more effective than two non species-specific probiotics to improve growth in Escherichia coli K88-challenged weaning pigs

The Partner from Wageningen University (additional contact person: Hauke Smidt, Hesselink van Suchtelenweg 4, 6703 CT, WAGENINGEN, NETHERLANDS, tel +31-3-17483102, isolated a Lactobacillus amylovorus -like strain 001 (LabAmy), from pigs fed different fibre sources that showed a good balanced micro-flora. We tested this strain as a probiotic in the diet of weaned piglets orally challenged with E. coli K88.

48 piglets, weaned at 21 days of age, were fed control diet or control diet plus 10 x 10 CFU Lactobacillus amylovorus/day (provided by Partner 3). Pigs were orally challenged with 10 x 10 CFU E. coli K88ac O148 (F4) on day 7, and sacrificed on day 13 or 14. Pigs supplemented with LabAmy grew faster (+ 74%, P<0.05), while did not show different feed intake, before and after challenge, compared with control pigs. Surprisingly, an increased number of days with diarrhoea were observed with the supplementation. Faecal excretion of total E.coli and E. coli K88 was not affected.

The length and the weight of small intestine, the pH in stomach, duodenum, jejunum, cecum and colon, the morphology of small intestine, and K88-specific IgA in blood, saliva and intestinal secretion did not change with the supplementation. The Lactobacillus amylovorus -like strain is a valid candidate for supplementation of weaning pig diets, in case of not optimal sanitary conditions in the farm. The same results were not obtained with Bifidobacterium animalis MB 05 and Lactobacillus rhamnosus GG. Furthermore Lactobacillus rhamnosus GG, strain not recovered usually in pigs, impaired growth performance.

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