Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

Co-cultures of human and pig enterocytes/lymphocytes as models to evaluate the effect of alternatives to in-feed antibiotics

Several alternatives to in-feed antibiotics, such as zinc oxide, glutamine, organic acids (Ca-formate, formic acid and Na-butyrate) and probiotics have been tested on two different types of co-cultures: Caco-2 cells co-cultured with human PBMC (peripheral blood mononuclear cells) and IPEC-1 cells co-cultured with lymphocytes isolated from pig mesenteric lymph nodes (MLN). The effect of all these alternatives on membrane permeability was measured.

The effect of zinc oxide (ZnO) against enterotoxigenic E. coli (ETEC) K88 induced cell damage was tested by measuring the transepithelial electrical resistance (TEER) and 14C-inulin transfer from apical to basolateral compartment. A protection against ETEC induced reduction of membrane permeability was observed already with a concentration of 0.2mM ZnO. Also formic acid (25-30mM) was able to protect against ETEC, whereas butyrate (up to 8mM) was ineffective.

Experiments on Caco-2 cells and human co-cultures treated with Bifidobacterium animalis and Lactobacillus casei GG showed that these probiotics did not prevent the ETEC induced permeability decrease, even with probiotic concentration 100 times higher than ETEC.

Experiments performed on porcine co-cultures infected with ETEC and treated with L. amylovorus-like and L. amylovorus DSMZ gave similar results to those obtained when IPEC-1 cells were cultured alone. In summary, the effects observed on TEER of co-cultures did not differ from that observed on intestinal cells cultured without lymphocytes.

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