Community Research and Development Information Service - CORDIS

Optimisation of immunoblotting of muscle specimens

The specificity and suitability of antibodies, muscle specimens and membrane preparations was evaluated. This included the testing of various monoclonal and polyclonal antibodies to muscle proteins involved in fibre contraction, the regulation of ion homeostasis and excitation-contraction coupling. Various tissues from animal models and humans biopsies were analysed and the suitability of subcellular fractionation procedures evaluated. Optimum visualization of immuno-decorated proteins was shown to occur with the enhanced chemiluminescence technique.

This knowledge will be used for the future analysis of normal, diseased and aged muscle samples. It is planed to perform a comprehensive comparison of the protein expression patterns between normal and aged fibres over the next three years.

Reported by

National University of Ireland
Maynooth, Co.
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