Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

Protocols for staining and counting of satellite cells in thick tissue sections

We have established protocols for the detection of satellite cells in thick muscle sections and establish sampling design of very rare structures as satellite cells are, starting from muscles of individual persons and ending with completely registered stacks of optical images captured by a confocal microscope. Here, satellite cells were defined from their association with M-Cadherin and their location beneath the basal lamina.

To avoid the biased counting of satellite cell profiles or myonuclear profiles used so far, which can lead to erroneous results, because the same satellite cells or the same myonuclei can be seen in several successive sections, we have introduced an unbiased counting method, the Disector.

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University of Ljubljana, Medical Faculty
Korytkova 2
1000 Ljubljana
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