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An analytical procedure, based on high performance liquid chromatography (HPLC) for the simultaneous detection of AAL toxins, produced by Alternata alternata f.sp. lycopersici, and fumonisins

The assay was set up to check the toxigenicity of A. alternata isolated from carrots and other umbelliferous plants and cultured on rice material. The fungus Alernaria alternata produce several mycotoxins harmful for humans and animals. The occurrence of Alternaria alternata on carrots produced in Europe is quite common. The determination of the mycotoxins profile of A. alternata isolated from carrots can help in predicting the risk of a possible mycotoxins contamination of the crop. It was previously reported that some strains of A. alternata were able to produce both fumonisin B1 and AAL toxins. A method was developed for the simultaneous determination of fumonisins (FB1, FB2) and AAL-toxins (TA, a mixture of two structural isomers TA1 and TA2) in inoculated rice cultures of A. alternata. Toxins were extracted from test samples with a mixture of water and acetonitrile. The filtered extract was diluted with a solution of potassium chloride and purified by solid phase extraction on a polymeric Oasis HLB column. The purified sample extract was submitted to pre-column derivatisation with OPA reagent and analysed by C18 reversed phase HPLC with fluorometric detection. Mean recoveries and repeatability for FB1, FB2 and TA were 89% (6%), 89% (9%) and 70% (23%), respectively. Quantification limits (signal to noise 6/1) were 0.1µg/g for TA and FB1 and 0.5µg/g for FB2.

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CNR Institute of Sciences of Food Production (ISPA)
Via Amendola 122/O
70125 BARI
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