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A protocol for testing organisms with antagonistic properties and/or plant extracts for biocontrol efficacy towards Alternaria radicina in infected carrot seeds

A protocol for testing efficacy of fungal and yeast antagonists in plant bioassays was developed. The test was based on:

- Carrot seeds naturally infected with Alternaria radicina and A. dauci.
- Coating infected seeds with a defined number of spores from potential antagonists.
- Assessment of plant emergence and seedling health in sand.

In a second screening the best isolates were further tested on seeds artificially inoculated with A. radicina using two inoculum levels of the pathogen. The method can be used to study and evaluate efficacy of biocontrol candidates and botanicals. Furthermore the bioassay offers a fast and low cost method to test carrot resistance to seedborne Alternaria spp.

Reported by

The Royal Veterinary and Agricultural University
40, Thorvaldsensvej
DK-1871 Frederiksberg C
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