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Microsatellite markers for genetic screening of the populations of the pine processionary moth (thaumetopoea pityocampa)

Six microsatellite markers were developed for the lepidopteran species Thaumetopoea pityocampa using an enrichment protocol before cloning. Five of them are already used in routine for population genetic studies on T. pityocampa, and four are also usable for T. wilkinsoni. The six markers are unlinked and show different polymorphism levels (from low to high). Development method and needed minimum technical details for use are described in an international journal of genetics (Molecular Ecology Notes). Information for synthesis and use of the needed PCR primers is also freely available from the associated international database (MENotes Database ID: 12587730, 12587732, 12587733, 12587735, 12587737). The original clone sequences (as references) are deposited in an international free database (Genbank, accession numbers: AY520912, AY520913, AY520914, AY520915, AY520916).

These molecular markers are of interest for population and ecological studies on pine processionary moths at different space and time scales (from broad to fine). They are excellent markers for studying:
(i) genetic diversity and population genetic structure,
(ii) gene flow, dispersal and migration related issues,
(iii) effective population size and demographic events,
(iv) parentage and relatedness.

The result allows to study recent population history and to infer some factors acting on it. Consequently, they could constitute a tool to address other applied questions related to PPM in the Mediterranean area, such as the origin of the expanding populations. The result could support the development of studies at a local scale in order to help forest managers or public authorities (e.g. regional councils) to deal with tree or public health concerns (colonization pathways, assessment of possible barriers to the expansion, identification of factors promoting the dispersal, risk of host plant shift and choice of planted tree species).

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INRA Orleans
45166 Olivet
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