Servizio Comunitario di Informazione in materia di Ricerca e Sviluppo - CORDIS

Immobilisation of RNA on nanofabricated electrodes

Methods have been developed to specifically immobilise and quantify the amount of functionalised oligonucleotides and RNA oligomers on the surface of both gold and reactive polymer modified electrodes. The reactive polymer can be either an activated ester of a poly (pyrrole) derivative or an amine terminated poly (pyrrole) derivative. In both cases a variety of coupling strategies can be used to selectively deposit different sequence oligos on to different electrodes within a lithographically patterned array (described in other another e-tip result). (The polymers are deposited onto lithographically patterned electrodes using electrochemical techniques.) Assessment of the specificity and degree of oligo and RNA immobilisation is performed using XPS and QCM techniques. Using these methods, optimisation of both solution parameters and conjugation strategies has been perform.

Having immobilised the oligonucleotides and RNA, it is then possible to immobilise on the modified electrode surface, gold nanoparticles that have been decorated with appropriate oligonucleotide and RNA motifs. This immobilisation takes place through the Mg2+ mediated association of structural motifs in the tertiary structure of specific RNA sequences.

Currently this work is being prepared for dissemination in scientific journals and is of potential use in the controlled creation of nanoscale wires and interconnections between nanoparticles having particular functionalities.

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University of Glasgow
Dept of Electronics, University of Glasgow
G12 8LT Glasgow
United Kingdom