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FP5

ACE Report Summary

Project ID: EVK1-CT-2001-00100
Funded under: FP5-EESD
Country: Italy

Analysis of estrogenic chemicals in mixtures

A very fast LC-MS method was developed for a rapid (max. 15 minutes analysis time), high-throughput determination of non steroidal, natural (genistein) and synthetic (octylphenol (OP), nonylphenol (NP), nonylphenol mono- and diethoxylates (NP1EO and NP2EO), nonylphenol monoethoxylate carboxylate (NP1EC)) endocrine disrupter compounds (EDCs) in water samples. MS detection is performed by using an Electrospray Interface (ESI) in positive or negative ionization mode. Obtained Method Detection limits (MDLs) are between 0.03µg/L for carboxylated nonylphenol monoethoxylate and 20µg/L for nonylphenol monoethoxylate. The method permits to markedly speed-up the analysis of EDCs mixtures because no extraction/clean up step is necessary, in comparison with traditional methods for the extraction of EDCs from water samples, which are time-consuming (approx. 6-8h/sample) even if fully automated. Possible applications of this method lie mainly in those situations where a large number of samples must be analysed in a short time, and lowest concentration level are in the 0.03-20µg/L level. Toxicity tests or biodegradation experiments could greatly benefit from this technique. In addition, the monitoring of wastewater could be performed by applying this protocol. The proposed method can be easily extended with minor modifications also to other EDCs and organic polar pollutants

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University of Venice, Environ Sci Dept
Calle Larga S. Marta 2137
30123 Venice
Italy
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