Wspólnotowy Serwis Informacyjny Badan i Rozwoju - CORDIS

Qualitative and quantitative analysis of dyer's greenweed

Refinement of the PDA HPLC method to distinguish between the flavonoid dyes weld and dyer's greenweed (DGW) was essential prior to analysis of historical and model samples. This was successfully achieved by improving the chromatographic separation of three characterising compounds - luteolin and apigenin (flavonoids present in both dyes) and genistein (present only in DGW).

With this improved PDA HPLC method, component ratios for five DGW model samples prepared by different dyeing processes were measured quantitatively. The respective ratios for genistein: luteolin: apigenin were found to vary between 9%:86%:5% (alum mordant, three overdyeings) and 48%:42%:9% (unmordanted, single dyeing). This indicated that the dyeing process varied the relative amounts of the three characteristic flavonoids for DGW even before ageing. This had been speculated, but not investigated, before the project. On the basis of this result and that from the dye's accelerated ageing study, it was concluded that identification of DGW is more reliable by qualitative PDA HPLC analysis than by quantitative analysis. The dye classification of historical tapestry samples by PDA HPLC was continuously reviewed in response to information from the PDA HPLC results from model samples and accelerated ageing studies.

Reported by

National Museums of Scotland
Royal Museum of Scotland Chambers Street
EH1 1JF Edinburgh
United Kingdom
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